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miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression

  • Authors:
    • Kun Duan
    • Yong‑Chao Ge
    • Xue‑Pei Zhang
    • Shu‑Yi Wu
    • Jin‑Shun Feng
    • Shi‑Lin Chen
    • Li Zhang
    • Zhi‑Hao Yuan
    • Chao‑Hong Fu

  • View Affiliations

  • Published online on: August 26, 2015     https://doi.org/10.3892/ol.2015.3645
  • Pages: 3223-3227
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

MicroRNA-34a (miR-34a) functions as a tumor suppressor gene and inhibits abnormal cell growth by regulating the expression of other genes. The role of miR‑34a in regulating sirtuin 1 (SIRT1) in prostate cancer remains unclear. The objective of the present study was to investigate the biological function and molecular mechanisms of miR‑34a regulation of SIRT1 in human prostate cancer samples and the human prostate cancer cell line, PC‑3. Fresh prostate tissues were obtained from patients, and the miR‑34a expression in prostate cancer tissues was measured using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). qPCR and western blotting were performed to assess the effects of miR‑34a overexpression on SIRT1 regulation in PC‑3 cells, and the cell growth was assessed by Cell Counting Kit‑8 (CCK‑8). Flow cytometry was used to assess the cell cycle status of the cells. The miR‑34a expression levels in prostate cancer tissues were significantly reduced compared with adjacent normal prostate tissues (P<0.05). SIRT1 expression levels in PC‑3 cells with over‑expression of miR‑34a were significantly reduced compared with those in the negative control (P<0.05). The over‑expression of miR‑34a inhibited PC‑3 cells growth and resulted in increased cell cycle arrest compared with the negative control (P<0.05). In conclusion, miR‑34a inhibits the human prostate cancer cell proliferation, in part, through the downregulation of SIRT1 expression.
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November-2015
Volume 10 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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  • Impact Factor: 2.5
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  • CiteScore: 5.7
  • CiteScore Rank: Q2: #139/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.574
  • SCImago Journal Rank (SJR): 0.644
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Spandidos Publications style
Duan K, Ge YC, Zhang XP, Wu SY, Feng JS, Chen SL, Zhang L, Yuan ZH and Fu CH: miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression. Oncol Lett 10: 3223-3227, 2015.
APA
Duan, K., Ge, Y., Zhang, X., Wu, S., Feng, J., Chen, S. ... Fu, C. (2015). miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression. Oncology Letters, 10, 3223-3227. https://doi.org/10.3892/ol.2015.3645
MLA
Duan, K., Ge, Y., Zhang, X., Wu, S., Feng, J., Chen, S., Zhang, L., Yuan, Z., Fu, C."miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression". Oncology Letters 10.5 (2015): 3223-3227.
Chicago
Duan, K., Ge, Y., Zhang, X., Wu, S., Feng, J., Chen, S., Zhang, L., Yuan, Z., Fu, C."miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression". Oncology Letters 10, no. 5 (2015): 3223-3227. https://doi.org/10.3892/ol.2015.3645