Effect of camptothecin on inducible nitric oxide synthase expression in the colon cancer SW480 cell line

Shen,Xiangdi; Chen,Jian; Qiu,Rong; Fan,Xingli; Xin,Ying

doi:10.3892/ol.2015.3658

Effect of camptothecin on inducible nitric oxide synthase expression in the colon cancer SW480 cell line

Authors:
- Xiangdi Shen
- Jian Chen
- Rong Qiu
- Xingli Fan
- Ying Xin
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Affiliations: Department of Basic Science, Zhejiang Medical College, Hangzhou, Zhejiang 310053, P.R. China, Department of General Surgery, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China
Published online on: September 2, 2015 https://doi.org/10.3892/ol.2015.3658
Pages: 3157-3160

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Abstract

As a topoisomerase I inhibitor, camptothecin (CPT) is regarded as an effective antitumor agent. In an attempt to search for its novel anticancer mechanism, the present study evaluated the effects of CPT on inducible nitric oxide synthase (iNOS) in the human colon cancer SW480 cell line when stimulated with lipopolysaccharide (LPS) and interleukin (IL)‑1β. The data indicated that CPT significantly decreased NO production. Consistent with these observations, the protein and mRNA expression levels of iNOS were inhibited by CPT in a dose‑dependent manner. Thus, the inhibitory effects of CPT on LPS/IL‑1β‑stimulated NO production were likely mediated via the inhibition of iNOS gene transcription. From these results, we propose that the inhibition of NO biosynthesis by CPT may partially underlie the efficacy of this antitumor agent.

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