miR‑339‑3p inhibits proliferation and metastasis of colorectal cancer

Zhou,Chang; Lu,Yenxia; Li,Xuenong

doi:10.3892/ol.2015.3661

miR‑339‑3p inhibits proliferation and metastasis of colorectal cancer

Authors:
- Chang Zhou
- Yenxia Lu
- Xuenong Li
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Affiliations: Department of Anatomy and Histology, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
Published online on: September 2, 2015 https://doi.org/10.3892/ol.2015.3661
Pages: 2842-2848
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs) serve important roles in regulating cancer cell proliferation and metastasis. The same hairpin RNA structure may produce mature products from each strand, termed miR‑5p and miR‑3p, which can bind different mRNAs. Previously, the present authors reported that miR‑339‑5p could inhibit cell proliferation and migration by targeting the 3'-untranslated region (3'-UTR) of PRL‑1 mRNA. The present study analyzed the expression, function and preliminary regulatory mechanism of miR‑339‑3p in colorectal cancer (CRC). The results of reverse transcription-quantitative polymerase chain reaction analysis demonstrated that miR‑339‑3p is downregulated in CRC specimens and highly invasive cell lines. Furthermore, the low‑level expression of miR‑339‑3p was significantly associated with lymph node metastasis in patients with CRC; however, reduced miR-339-3p expression was not associated with age, gender or the differentiation status of the tumor. Overexpression of miR‑339‑3p was sufficient to suppress tumor growth and metastasis in vitro. In addition, the present study demonstrated that unlike miR-339-5p, PRL‑1 expression was not regulated by miR‑339‑3p. The findings of the present study indicate that miR‑339‑5p and miR‑339‑3p may target different mRNA. The target gene of miR‑339‑3p requires future identification.

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