Rapid and reliable detection of CD44 variants in gastric carcinoma using a nested reverse transcription‑polymerase chain reaction

Wang,Yan; Liu,Yuhong; Xiao,Bai

doi:10.3892/ol.2015.3664

Rapid and reliable detection of CD44 variants in gastric carcinoma using a nested reverse transcription‑polymerase chain reaction

Authors:
- Yan Wang
- Yuhong Liu
- Bai Xiao
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Affiliations: Department of Gastroenterology, Beijing Chao Yang Hospital Affiliated to Capital Medical University, Beijing 100020, P.R. China, Department of Experimental Medicine, Beijing Chao Yang Hospital Affiliated to Capital Medical University, Beijing 100020, P.R. China
Published online on: September 2, 2015 https://doi.org/10.3892/ol.2015.3664
Pages: 2962-2966

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Abstract

The present study aimed to establish a rapid and reliable method for detecting the expression of cluster of differentiation 44 variant (CD44v) in gastric carcinoma, and to investigate the significance of CD44v in gastric carcinoma. Using a nested reverse transcription‑polymerase chain reaction (RT‑PCR) technique, the expression of CD44v and CD44v8‑10 was analyzed in gastric cancer tissues (128 cases), precancerous lesions (19 cases of atypical hyperplasia and 6 cases of intestinal metaplasia) and corresponding adjacent non‑cancerous tissues (153 cases). The tumor and non‑cancerous biopsy samples of 153 patients were analyzed using nested RT‑PCR. All the PCR products included bands at 482 bp, demonstrating positive CD44 expression. By contrast, the CD44v band (>600 bp) was observed in 132/153 total tumor samples (86.3%), including 114/128 gastric cancer samples (89.1%), 16/19 atypical hyperplasia samples (84.2%) and 2/6 intestinal metaplasia samples (33.3%). However, 18/153 non‑cancerous tissues samples (11.8%) exhibited a CD44v band. Thus, CD44v expression was significantly higher in gastric cancer tissues and precancerous lesions compared with that of adjacent non‑cancerous tissues (P<0.05). Furthermore, there was a significant difference in CD44v8‑10 expression detected between gastric cancer and adjacent non‑cancerous tissue samples (P<0.05). Among the 25 patients with precancerous lesions, 8/19 atypical hyperplasia cases and 1/6 intestinal metaplasia cases were positive for CD44v8‑10 expression. The difference in the CD44v8‑10 expression rate among the various pathological types of gastric cancer (n=128) cases was not significant (P>0.05). Additionally, immunohistochemical analysis identified CD44v positivity (++) in 59/76 (77.6%) cases of gastric cancer and 5/12 (41.1%) cases of atypical hyperplasia. The CD44v and CD44v8‑10 PCR products were confirmed by sequencing analysis. The results of the present study indicated that nested RT‑PCR technology may be exploited as a method for gastric carcinoma diagnosis.

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