JAK/STAT3 signaling pathway mediates endothelial‑like differentiation of immature dendritic cells

Jin,Guoguo; Zhao,Jimin; Yang,Yi; Liu,Kangdong; Jiang,Yanan; Zhang,Xiaoyan; Zhang,Yanyan; Huang,Youtian; Lu,Jing; Dong,Ziming

doi:10.3892/ol.2015.3752

JAK/STAT3 signaling pathway mediates endothelial‑like differentiation of immature dendritic cells

Authors:
- Guoguo Jin
- Jimin Zhao
- Yi Yang
- Kangdong Liu
- Yanan Jiang
- Xiaoyan Zhang
- Yanyan Zhang
- Youtian Huang
- Jing Lu
- Ziming Dong
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Affiliations: Department of Pathophysiology, Basic Medical College, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China
Published online on: September 25, 2015 https://doi.org/10.3892/ol.2015.3752
Pages: 3471-3477
Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Endothelial‑like differentiation (ELD) of dendritic cells (DCs) is a poorly understood phenomenon. The present study evaluated the effect on the ELD of DCs by using human esophageal squamous cell carcinoma (ESCC) cells with high or poor differentiation. The results demonstrated that KYSE450 (highly differentiated) and KYSE70 (poorly differentiated) cell supernatants induce the differentiation of immature DCs (iDCs), derived from healthy adult volunteers, away from the DC pathway and towards an endothelial cell (EC) fate. This effect was strongest in the cells treated with the KYSE70 supernatant. During the ELD of iDCs, sustained activation of JAK (janus tyrosine kinase)/STAT3 (signal transducer and activator of transcription 3) signaling was detected. Incubation of iDCs with the JAK inhibitor, AG490 blocked JAK/STAT3 phosphorylation and iDC differentiation. These results suggested that the JAK/STAT3 signaling pathway mediates ELD of iDCs. Furthermore, the poorly differentiated ESCC cells may have a greater effect on the ELD of iDCs than highly differentiated ESCC cells.

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