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Article

Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue

  • Authors:
    • Huiyong Jiang
    • Xiaoyan Bai
    • Fanjun Meng
    • Xuefeng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110840, P.R. China, Division of Nephrology, Nanfang Hospital, Southern Medical University and Guangdong Provincial Institute of Nephrology, Guangzhou, Guangdong 510515, P.R. China, Department of Gastroenterology, 202nd Hospital of the People's Liberation Army, Shenyang, Liaoning 110003, P.R. China
  • Pages: 3581-3584
    |
    Published online on: September 29, 2015
       https://doi.org/10.3892/ol.2015.3757
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Abstract

Thyroid transcription factor-1 (TTF-1) reportedly possesses oncogenic and suppressive roles within the same tumor type and may play a dual function in the progression of lung cancer. Immunohistochemistry (IHC) and mRNA in situ hybridization (ISH) are commonly used methods for detecting protein or mRNA expression. The present study compared the concordance rate of the two methods in the evaluation of thyroid transcription factor‑1 (TTF‑1) expression in non‑small cell lung carcinoma (NSCLC) using tissue microarray‑based IHC and mRNA ISH. TTF‑1 protein and mRNA expression levels were examined in 196 cases of NSCLC. The IHC and mRNA ISH agreement was 91.3% (179/196), and near‑perfect agreement was observed between the two methods (κ‑coefficient, 0.848). There was no significant difference between IHC and mRNA ISH, as analyzed by the McNemar‑Bowker test (P=0.219). The present findings proved that IHC is comparable to mRNA ISH for evaluating TTF‑1 expression in NSCLC. These two methods can be used to detect TTF-1 expression in future studies.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang H, Bai X, Meng F and Zhang X: Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue. Oncol Lett 10: 3581-3584, 2015.
APA
Jiang, H., Bai, X., Meng, F., & Zhang, X. (2015). Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue. Oncology Letters, 10, 3581-3584. https://doi.org/10.3892/ol.2015.3757
MLA
Jiang, H., Bai, X., Meng, F., Zhang, X."Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue". Oncology Letters 10.6 (2015): 3581-3584.
Chicago
Jiang, H., Bai, X., Meng, F., Zhang, X."Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue". Oncology Letters 10, no. 6 (2015): 3581-3584. https://doi.org/10.3892/ol.2015.3757
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang H, Bai X, Meng F and Zhang X: Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue. Oncol Lett 10: 3581-3584, 2015.
APA
Jiang, H., Bai, X., Meng, F., & Zhang, X. (2015). Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue. Oncology Letters, 10, 3581-3584. https://doi.org/10.3892/ol.2015.3757
MLA
Jiang, H., Bai, X., Meng, F., Zhang, X."Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue". Oncology Letters 10.6 (2015): 3581-3584.
Chicago
Jiang, H., Bai, X., Meng, F., Zhang, X."Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue". Oncology Letters 10, no. 6 (2015): 3581-3584. https://doi.org/10.3892/ol.2015.3757
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