Demethylation effects of elemene on the GSTP1 gene in HCC cell line QGY7703

  • Authors:
    • Baoqiang Wu
    • Yong Jiang
    • Feng Zhu
    • Donglin Sun
    • Hongjun Huang
  • View Affiliations

  • Published online on: February 18, 2016     https://doi.org/10.3892/ol.2016.4243
  • Pages: 2545-2551
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Abstract

The present study aimed to investigate elemene's effects on cell proliferation, apoptosis, and the cell cycle in the hepatocellular carcinoma (HCC) cell line, QYG7703, and to investigate GSTP1 gene methylation change in QGY7703 cells after being treated with elemene to explore whether elemene reversed the abnormal GSTP1 gene methylation. QGY7703 cells were treated with different elemene concentrations. Cell proliferation was measured with MTT assay, cell apoptosis and cell cycle were analyzed by flow cytometry, and GSTP1 gene methylation was analyzed by methlation‑specific polymerase chain reaction. The cells' apoptotic rate increased dose‑dependently with elemene concentration, and the difference was statistically significant (P<0.05). Elemene treatment arrested the cells in S phase, and thus the percentage of cells in G1 phase decreased while the cells in S phase increased dose‑dependently, and the difference was statistically significant compared to the control group (P<0.05). All QGY7703 cells were identified to contain GSTP1 gene methylation before being treated with elemene and the methylation state decreased after treatment. In the present study, elemene induced cell apoptosis, inhibited the cell cycle, and reversed GSTP1 gene methylation in QGY7703 cells.
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April-2016
Volume 11 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wu B, Jiang Y, Zhu F, Sun D and Huang H: Demethylation effects of elemene on the GSTP1 gene in HCC cell line QGY7703. Oncol Lett 11: 2545-2551, 2016
APA
Wu, B., Jiang, Y., Zhu, F., Sun, D., & Huang, H. (2016). Demethylation effects of elemene on the GSTP1 gene in HCC cell line QGY7703. Oncology Letters, 11, 2545-2551. https://doi.org/10.3892/ol.2016.4243
MLA
Wu, B., Jiang, Y., Zhu, F., Sun, D., Huang, H."Demethylation effects of elemene on the GSTP1 gene in HCC cell line QGY7703". Oncology Letters 11.4 (2016): 2545-2551.
Chicago
Wu, B., Jiang, Y., Zhu, F., Sun, D., Huang, H."Demethylation effects of elemene on the GSTP1 gene in HCC cell line QGY7703". Oncology Letters 11, no. 4 (2016): 2545-2551. https://doi.org/10.3892/ol.2016.4243