Open Access

siRNA targeting YAP gene inhibits gastric carcinoma growth and tumor metastasis in SCID mice

  • Authors:
    • Zhou Zhou
    • Jin‑Shui Zhu
    • Cai‑Ping Gao
    • Liang‑Ping Li
    • Chao Zhou
    • Han Wang
    • Xiao‑Gang Liu
  • View Affiliations

  • Published online on: March 9, 2016     https://doi.org/10.3892/ol.2016.4319
  • Pages: 2806-2814
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Yes-associated protein (YAP) is constitutively activated in numerous types of cancer, including gastric carcinoma. The aim of the present study was to investigate the effects of YAP silencing on proliferation, apoptosis, metastasis and angiogenesis in a gastric orthotopic implantation cancer model of severe combined immunodeficiency mice. Small‑hairpin RNA (shRNA) targeting the YAP gene was employed to inhibit YAP expression. SGC7901 cells transfected with YAP shRNA demonstrated significantly decreased gastric cancer growth and metastasis in the orthotopic implantation mouse model. Silencing of YAP additionally promoted tumor cell apoptosis, and inhibited tumor cell proliferation and angiogenesis. Notably, YAP shRNA also downregulated the expression of TEA domain family member 1, cyclinD1, vascular endothelial growth factor and fibroblast growth factor‑2. The results of the present study suggested that YAP may have a significant role in the proliferation, metastasis and angiogenesis of gastric cancer. RNA interference‑mediated silencing of YAP may provide an opportunity to develop a novel treatment strategy for gastric cancer.
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April-2016
Volume 11 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhou Z, Zhu JS, Gao CP, Li LP, Zhou C, Wang H and Liu XG: siRNA targeting YAP gene inhibits gastric carcinoma growth and tumor metastasis in SCID mice. Oncol Lett 11: 2806-2814, 2016.
APA
Zhou, Z., Zhu, J., Gao, C., Li, L., Zhou, C., Wang, H., & Liu, X. (2016). siRNA targeting YAP gene inhibits gastric carcinoma growth and tumor metastasis in SCID mice. Oncology Letters, 11, 2806-2814. https://doi.org/10.3892/ol.2016.4319
MLA
Zhou, Z., Zhu, J., Gao, C., Li, L., Zhou, C., Wang, H., Liu, X."siRNA targeting YAP gene inhibits gastric carcinoma growth and tumor metastasis in SCID mice". Oncology Letters 11.4 (2016): 2806-2814.
Chicago
Zhou, Z., Zhu, J., Gao, C., Li, L., Zhou, C., Wang, H., Liu, X."siRNA targeting YAP gene inhibits gastric carcinoma growth and tumor metastasis in SCID mice". Oncology Letters 11, no. 4 (2016): 2806-2814. https://doi.org/10.3892/ol.2016.4319