Expression and regulatory effects of microRNA-182 in osteosarcoma cells: A pilot study

Bian,Dong‑Lin; Wang,Xue‑Mei; Huang,Kun; Zhai,Qi‑Xi; Yu,Gui‑Bo; Wu,Cheng‑Hua

doi:10.3892/ol.2016.4375

Expression and regulatory effects of microRNA-182 in osteosarcoma cells: A pilot study

Authors:
- Dong‑Lin Bian
- Xue‑Mei Wang
- Kun Huang
- Qi‑Xi Zhai
- Gui‑Bo Yu
- Cheng‑Hua Wu
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Affiliations: Department of Ultrasound, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Key Laboratory of Diagnosis and Interventional Therapy of Liaoning Province, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Radiology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
Published online on: March 23, 2016 https://doi.org/10.3892/ol.2016.4375
Pages: 3040-3048
Copyright: © Bian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to evaluate the expression level of microRNA-182 (miRNA-182) in human osteosarcoma (OS) MG-63 cells and OS tissues, and to elucidate the effect of miRNA-182 on the biological activity of tumors. In the present study, the expression of miRNA‑182 in human OS MG‑63 cells, OS tissues and normal osteoblast hFOB1.19 cells was determined using quantitative polymerase chain reaction. Subsequently, a miRNA‑182 mimic and inhibitor were utilized to regulate the expression level of this miRNA in MG‑63 cells. Cell viability and proliferation were examined using cell counting kit‑8 assays, and cell apoptosis was detected by flow cytometry. Cell invasion and migration assays were performed using Transwell chambers to analyze the biological functions of miRNA‑182 in vitro. The present study demonstrated that the expression level of miRNA‑182 in MG‑63 cells and OS tissues was significantly increased compared with the hFOB1.19 cell line (P<0.05). The present study successfully performed cell transfections of miRNA‑182 inhibitor and miRNA‑182 mimic into MG‑63 cells and achieved the desired transfection efficiency. The present study confirmed that upregulation of miRNA‑182 promotes cell apoptosis and inhibits cell viability, proliferation, invasion and migration. The present findings additionally demonstrated that miRNA‑182 is a tumor suppressor gene in OS. Therefore, regulating the expression of miRNA-182 may affect the biological behavior of OS cells, which suggests a potential role for miRNA-182 in molecular therapy for malignant tumors.

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