Knockdown of LMP1-induced miR-155 sensitizes nasopharyngeal carcinoma cells to radiotherapy in vitro

Wang,Yusheng; Sun,Le

doi:10.3892/ol.2016.4400

Knockdown of LMP1-induced miR-155 sensitizes nasopharyngeal carcinoma cells to radiotherapy in vitro

Authors:
- Yusheng Wang
- Le Sun
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Affiliations: Department of Otolaryngology, Head and Neck Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: March 31, 2016 https://doi.org/10.3892/ol.2016.4400
Pages: 3451-3456

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Abstract

The present study aimed to confirm the promotion of microRNA (miR)‑155 expression by latent membrane protein 1 (LMP1), and to recognize the oncogenic role of LMP1 and LMP1-promoted miR‑155 in nasopharyngeal carcinoma (NPC), particularly the influence of miR-155 knockdown on the radiosensitivity of CNE‑2 cells. Following the regulation of the levels of LMP1 or miR-155 and/or subsequent to radiation treatment, the proliferation ability of CNE‑2 cells was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation and Cell Counting Kit‑8 assays. The results demonstrated that miR‑155 was upregulated by overexpression of LMP1 in CNE‑2 cells, and LMP1 overexpression and miR‑155 mimic transfection increased CNE‑2 cell proliferation, whereas miR‑155 knockdown attenuated the promotion of CNE-2 cell growth induced by LMP1 overexpression. Furthermore, knockdown of miR‑155 enhanced the radiosensitivity of CNE‑2 cells. In conclusion, the present study confirmed the oncogenic role of miR‑155 in NPC, and demonstrated that knockdown of miR‑155 inhibited the growth of NPC cells and sensitized NPC cells to radiotherapy.

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