miR-20b downregulates polymerases κ and θ in XP-V tumor cells

Guo,Jia; Jiang,Zheng; Li,Xiangru; Wang,Xi; Xiao,Yan

doi:10.3892/ol.2016.4447

miR-20b downregulates polymerases κ and θ in XP-V tumor cells

Authors:
- Jia Guo
- Zheng Jiang
- Xiangru Li
- Xi Wang
- Yan Xiao
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Affiliations: Department of Endodontics, Oral Medical Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Endodontics, Xiamen Stomatological Hospital, Xiamen, Fujian 361004, P.R. China
Published online on: April 18, 2016 https://doi.org/10.3892/ol.2016.4447
Pages: 3790-3794

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Abstract

XP-V is a subtype of Xeroderma pigmentosum diseases with typical pigmentation and cancers in sun-exposed regions. The present study investigated the role of microRNA-20b (miR-20b) in the imbalance of polymerase expression levels in XP‑V tumor cells. Following software prediction results, certain miRNAs were chosen as candidate regulators for the observed imbalance in polymerases in XP‑V tumor cells. Reverse transcription‑quantitative polymerase chain reaction and western blot were used to test candidate miRNAs for their ability to reduce the expression of these polymerases. A luciferase reporter assay was used to further verify the western blot results. Polymerases κ and θ were expressed at lower levels in XP‑V tumor cells compared to normal control cells. A positive correlation was demonstrated between miR-20b and polymerases κ and θ. It was also demonstrated that a proportion of miRNAs had no effect on polymerases κ and θ, despite the software predicting that these miRNAs would target these two polymerases. Therefore, miR‑20b may be responsible for the low expression levels of polymerase κ and θ in XP‑V tumor cells, which accelerated mismatch in DNA replication repairing.

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