Open Access

Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer

  • Authors:
    • Yu‑Yi Hou
    • Jyun‑Jie You
    • Cheng‑Mei Yang
    • Hung‑Wei Pan
    • Hung‑Chih Chen
    • Jang‑Hwa Lee
    • Yaoh‑Shiang Lin
    • Huei‑Han Liou
    • Pei‑Feng Liu
    • Chao‑Chuan Chi
    • Luo‑Ping Ger
    • Kuo‑Wang Tsai
  • View Affiliations

  • Published online on: April 25, 2016     https://doi.org/10.3892/ol.2016.4491
  • Pages: 4013-4021
  • Copyright: © Hou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNAs (miRs) are a class of small endogenous non-coding RNAs of ~21-24 nucleotides in length. Previous studies have indicated that miR-196b has either an oncogenic or tumor-suppressive function in various types of cancer. However, the biological role of miR-196b in oral squamous cell carcinoma (OSCC) remains unclear. In the present study, the expression levels of miR‑196b were examined in oral cancer tissues and corresponding adjacent normal tissues from 69 OSCC patients using stem‑loop reverse transcription-quantitative polymerase chain reaction. The results indicated that miR-196b was significantly overexpressed in OSCC tissues compared with the corresponding adjacent normal tissue samples (64 of 69, 92.7%, P<0.001). Analysis of the methylation status of the miR‑196b gene indicated more frequent hypomethylation of the CpG islands located upstream of the miR‑196b gene in the OSCC tissues than in the adjacent normal tissues (32 of 69, 46.3%), and the methylation status of miR‑196b correlated inversely with its expression levels. Furthermore, the unmethylated status of the miR‑196b promoter correlated with poor disease-specific survival in OSCC patients (P=0.035). Functional analysis revealed that ectopic miR‑196b expression promoted oral cancer cell migration and invasion abilities, and that silencing of miR‑196b could abrogate in vitro migration and invasion of oral cancer cells. Collectively, the present findings indicate that the epigenetic regulation of miR-196b expression plays a crucial role in modulating cell migration and invasion during OSCC progression, and thus may serve as a potential prognosis marker or therapeutic target for OSCC.
View Figures
View References

Related Articles

Journal Cover

June-2016
Volume 11 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hou YY, You JJ, Yang CM, Pan HW, Chen HC, Lee JH, Lin YS, Liou HH, Liu PF, Chi CC, Chi CC, et al: Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer. Oncol Lett 11: 4013-4021, 2016
APA
Hou, Y., You, J., Yang, C., Pan, H., Chen, H., Lee, J. ... Tsai, K. (2016). Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer. Oncology Letters, 11, 4013-4021. https://doi.org/10.3892/ol.2016.4491
MLA
Hou, Y., You, J., Yang, C., Pan, H., Chen, H., Lee, J., Lin, Y., Liou, H., Liu, P., Chi, C., Ger, L., Tsai, K."Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer". Oncology Letters 11.6 (2016): 4013-4021.
Chicago
Hou, Y., You, J., Yang, C., Pan, H., Chen, H., Lee, J., Lin, Y., Liou, H., Liu, P., Chi, C., Ger, L., Tsai, K."Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer". Oncology Letters 11, no. 6 (2016): 4013-4021. https://doi.org/10.3892/ol.2016.4491