Knockdown of cathepsin L sensitizes ovarian cancer cells to chemotherapy

  • Authors:
    • Hongmei Zhang
    • Luosheng Zhang
    • Lixia Wei
    • Xingwang Gao
    • Li Tang
    • Wei Gong
    • Na Min
    • Li Zhang
    • Yawei Yuan
  • View Affiliations

  • Published online on: April 26, 2016     https://doi.org/10.3892/ol.2016.4494
  • Pages: 4235-4239
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Abstract

Ovarian cancer is a leading gynecological malignancy associated with high mortality. The development of acquired drug resistance is the primary cause of chemotherapy failure in the treatment of ovarian cancer. To examine the mechanism underlying paclitaxel resistance in ovarian cancer and attempt to reverse it, the present study induced a TAX‑resistant ovarian cancer cell line, SKOV3/TAX. Cathepsin L (CTSL) has been found to be overexpressed in ovarian cancer. The aim of the present study was to investigate the possible involvement of CTSL in the development of TAX resistance in ovarian cancer. CTSL expression was knocked down in SKOV3 ovarian cancer cells and their phenotypic changes were analyzed. The effects of silenced CTSL on the resistant cell line were investigated by proliferation and apoptosis analysis compared with control SKOV3 cells. CTSL was more highly expressed in SKOV3/TAX cells compared with SKOV3 cells. Paclitaxel treatment downregulated the expression of CTSL in SKOV‑3 but not in the paclitaxel‑resistant SKOV3/TAX cells. CTSL small hairpin RNA (shRNA) knockdown significantly potentiated apoptosis induced by paclitaxel compared with SKOV3/TAX cells transfected with control shRNA, suggesting that CTSL contributes to paclitaxel resistance in ovarian cancer cells and that CTSL silencing can enhance paclitaxel‑mediated cell apoptosis. Thus, CTSL should be explored as a candidate of therapeutic target for modulating paclitaxel sensitivity in ovarian cancer.
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June-2016
Volume 11 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang H, Zhang L, Wei L, Gao X, Tang L, Gong W, Min N, Zhang L and Yuan Y: Knockdown of cathepsin L sensitizes ovarian cancer cells to chemotherapy. Oncol Lett 11: 4235-4239, 2016.
APA
Zhang, H., Zhang, L., Wei, L., Gao, X., Tang, L., Gong, W. ... Yuan, Y. (2016). Knockdown of cathepsin L sensitizes ovarian cancer cells to chemotherapy. Oncology Letters, 11, 4235-4239. https://doi.org/10.3892/ol.2016.4494
MLA
Zhang, H., Zhang, L., Wei, L., Gao, X., Tang, L., Gong, W., Min, N., Zhang, L., Yuan, Y."Knockdown of cathepsin L sensitizes ovarian cancer cells to chemotherapy". Oncology Letters 11.6 (2016): 4235-4239.
Chicago
Zhang, H., Zhang, L., Wei, L., Gao, X., Tang, L., Gong, W., Min, N., Zhang, L., Yuan, Y."Knockdown of cathepsin L sensitizes ovarian cancer cells to chemotherapy". Oncology Letters 11, no. 6 (2016): 4235-4239. https://doi.org/10.3892/ol.2016.4494