miR-494 inhibits ovarian cancer cell proliferation and promotes apoptosis by targeting FGFR2

Zhao,Xiaojuan; Zhou,Yun; Chen,Yu; Yu,Feng

doi:10.3892/ol.2016.4527

miR-494 inhibits ovarian cancer cell proliferation and promotes apoptosis by targeting FGFR2

Authors:
- Xiaojuan Zhao
- Yun Zhou
- Yu Chen
- Feng Yu
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Affiliations: Department of Gynecology and Obstetrics, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi, Jiangsu 214000, P.R. China
Published online on: May 5, 2016 https://doi.org/10.3892/ol.2016.4527
Pages: 4245-4251

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Abstract

MicroRNAs (miRs) have been reported to be key regulators in numerous types of cancer. The aim of the present study was to investigate the role of miR‑494 in ovarian cancer. Expression of miR‑494 was analyzed in ovarian cancer tissues and cell lines by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). miR‑494 mimic or negative control was transiently transfected into A2780 and SKOV3 cell lines. A cell counting kit‑8 assay was performed to assess the effects of miR‑494 on cell proliferation, and flow cytometry was used to evaluate the apoptotic rate. The target gene of miR‑494 was detected by luciferase assay. Expression of fibroblast growth factor receptor 2 (FGFR2) was identified using RT‑qPCR and western blotting. In the present study, decreased expression of miR‑494 was observed in ovarian cancer samples and cell lines. Overexpression of miR‑494 inhibited ovarian cancer cell proliferation by inducing apoptosis. Additional investigation indicated that FGFR2 was a direct target of miR‑494. Taken together, the results of the present study suggested that miR-494 suppressed ovarian cancer cell proliferation by inducing apoptosis via targeting FGFR2.

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