miR-10b promotes invasion by targeting HOXD10 in colorectal cancer

Wang,Yunfeng; Li,Zhen; Zhao,Xuhong; Zuo,Xiaoming; Peng,Zhihai

doi:10.3892/ol.2016.4628

miR-10b promotes invasion by targeting HOXD10 in colorectal cancer

Authors:
- Yunfeng Wang
- Zhen Li
- Xuhong Zhao
- Xiaoming Zuo
- Zhihai Peng
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Affiliations: Department of General Surgery, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China, Department of Central Experiment Laboratory, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China, Department of Pathology, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China, Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, P.R. China
Published online on: May 25, 2016 https://doi.org/10.3892/ol.2016.4628
Pages: 488-494
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Studies have shown that homeobox D10 (HOXD10) is the target gene of microRNA-10b (miR-10b) and is closely associated with the inhibition of cell migration and invasion. Ras homolog family member C (RhoC) has been reported to promote tumor metastasis in various types of cancer. The effect of miR‑10b on colorectal cancer (CRC) metastasis and the associated molecular mechanisms remain elusive. The present study aimed to investigate whether miR‑10b could promote invasion by targeting HOXD10 in CRC by exploring the association between miR‑10b and HOXD10 expression in CRC patients. The findings revealed that miR‑10b levels were elevated in the CRC specimens and significantly correlated with advanced clinical stage and lymph node metastasis. In addition, HOXD10 was a direct target of miR‑10b, and the increased expression of RhoC and downregulation of HOXD10 correlated with the increased expression level of miR‑10b. HOXD10 protein level was also markedly attenuated in lymph node metastasis‑positive tumor tissues compared with lymph node metastasis‑free tumor tissues. These findings suggest that miR‑10b may stimulate the upregulation of RhoC through targeting HOXD10, thus promoting the invasion and migration in CRC tumor.

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