O‑GlcNAcylation enhances anaplastic thyroid carcinoma malignancy

Cheng,Yu; Li,Honglun; Li,Jianlin; Li,Jisheng; Gao,Yan; Liu,Baodong

doi:10.3892/ol.2016.4647

O‑GlcNAcylation enhances anaplastic thyroid carcinoma malignancy

Authors:
- Yu Cheng
- Honglun Li
- Jianlin Li
- Jisheng Li
- Yan Gao
- Baodong Liu
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Affiliations: Department of Medical Oncology, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai, Shandong 264000, P.R. China, Department of Radiology, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai, Shandong 264000, P.R. China, Department of Radiology, Penglai Hospital of Traditional Chinese Medicine, Penglai, Shandong 265600, P.R. China, Department of Dermatology, Yankuang Group General Hospital, Zoucheng, Shandong 273500, P.R. China, Department of Ultrasound, Yankuang Group General Hospital, Zoucheng, Shandong 273500, P.R. China
Published online on: May 30, 2016 https://doi.org/10.3892/ol.2016.4647
Pages: 572-578

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Abstract

O-linked N-acetylglucosamine (O-GlcNAc) glycosylation (O-GlcNAcylation), a dynamic post-translational modification of nuclear and cytoplasmic proteins, may have a critical role in the regulation of biological cell processes and human cancer. O‑GlcNAcylation is dynamically regulated by O‑GlcNAc transferase (OGT) and O‑GlcNAc hydrolase (OGA). Accumulating evidence suggests that O‑GlcNAcylation is involved in a variety of types of human cancer. However, the exact role of O‑GlcNAcylation in tumor pathogenesis or progression remains to be established. Computed tomography scans of patients with anaplastic thyroid carcinoma (ATC) reveal a rapid growth rate and invasion. The present study demonstrated that O‑GlcNAcylation accelerates the progression of ATC. The global O‑GlcNAc level of intracellular proteins was increased by overexpression of OGT or downregulation of OGA activity with the specific inhibitor Thiamet‑G. By contrast, the global O‑GlcNAc level was decreased by silencing of OGT. MTT assay indicated that O‑GlcNAcylation significantly promotes cell proliferation. Furthermore, O‑GlcNAcylation enhanced cellular biological functions, such as colony formation ability, migration and invasion, of ATC cells in vitro. The findings of the present study suggest that O‑GlcNAcylation is associated with malignant properties of thyroid cancer, and may be a potential target for the diagnosis and treatment of thyroid cancer.

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