p21 participates in the regulation of anaplastic thyroid cancer cell proliferation by miR-146b

Wang,Shiyang; Chen,Yangjing; Bai,Yanxia

doi:10.3892/ol.2016.4874

p21 participates in the regulation of anaplastic thyroid cancer cell proliferation by miR-146b

Authors:
- Shiyang Wang
- Yangjing Chen
- Yanxia Bai
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Affiliations: Department of Otorhinolaryngology ‑ Head and Neck Surgery, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
Published online on: July 18, 2016 https://doi.org/10.3892/ol.2016.4874
Pages: 2018-2022

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Abstract

Anaplastic thyroid carcinoma (ATC) originates from completely undifferentiated cells, and is the most lethal type of thyroid-derived tumor. Numerous microRNAs have significant roles in tumorigenesis by targeting relevant genes. The role of microRNA 146b (miR-146b) in ATC remains to be elucidated. In order to characterize the role of miR-146b in ATC, overexpression or interference of miR‑146b was induced in ATC cell lines, and cell proliferation and migration were evaluated. The potential targets of miR‑146b were searched in the Gene Expression Omnibus database for ATC and matched non‑tumor control samples. The expression level of potential targets was detected following overexpression or interference of miR-146b in ATC cell lines. In the present study, cell proliferation was promoted when overexpression of miR‑146b was induced in ATC, and inhibited when interference of miR‑146b was induced, which indicated that miR‑146b affects the proliferation of ATC cells in vitro. In addition, cell migration of ATC was also affected by miR‑146b. During the search for potential targets of miR‑146b in ATC, p21 (also known as p21Waf1/Cip1 or CDKN1A) was noted for its role in cell cycle progression and tumor pathogenesis. The expression level of p21 was influenced by the level of miR‑146b, and the results of the present study demonstrated that the level of p21 was increased when FRO cells were transformed with miR‑146b mimic, and p21 was downregulated when FRO cells transformed with anti-miR-146b. In conclusion, p21 may participate in the regulation of ATC cell proliferation by miR‑146b.

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