Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells

Wang,Shuhua; Tian,Qingqing; An,Fang

doi:10.3892/ol.2016.4898

Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells

Authors:
- Shuhua Wang
- Qingqing Tian
- Fang An
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Affiliations: Department of Pharmacy, Hebei North University, Zhangjiakou, Hebei 075000, P.R. China, Graduate Faculty, Hebei North University, Zhangjiakou, Hebei 075000, P.R. China
Published online on: July 21, 2016 https://doi.org/10.3892/ol.2016.4898
Pages: 1705-1710
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF‑7 cells and observed clear inhibitory effects within the concentration range of 0.0991‑1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl‑2 and NF‑κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.

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