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Article

Rescuing defective tumor‑infiltrating T‑cell proliferation in glioblastoma patients

  • Authors:
    • Song Han
    • Enlong Ma
    • Xiaonan Wang
    • Chunyong Yu
    • Tao Dong
    • Wen Zhan
    • Xuezhong Wei
    • Guobiao Liang
    • Sizhe Feng
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, General Hospital of Shenyang Military Area Command of the Chinese People's Liberation Army, Shenyang, Liaoning 110016, P.R. China, Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P.R. China, Lingbin Biotechnology Research Center, Jinan, Shandong 250100, P.R. China
  • Pages: 2924-2929
    |
    Published online on: August 3, 2016
       https://doi.org/10.3892/ol.2016.4944
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Abstract

Primary glioblastoma (GBM) is the most prevalent brain cancer, with fast progression and a poor prognosis. Current treatment options are unable to fully manage GBM since it is highly resistant to radiation and chemotherapy, and it cannot be completely removed by surgery. Thus, immunotherapeutic strategies utilizing tumor‑infiltrating T cells have been investigated. In the present study, the T‑cell response in GBM patients was examined in resected tumor samples and peripheral blood samples by flow cytometry. It was found that tumor‑infiltrating T cells represented a rare population in all tumor cells, and were more refractory to anti‑cluster of differentiation 3 (CD3) stimulation than their peripheral blood counterparts. A number of strategies were then assessed to boost tumor‑infiltrating T‑cell proliferation, and it was found that pre‑incubation with 20 U/ml interleukin (IL)‑2, as well as sequestration of IL‑10 in culture, improved tumor T‑cell proliferation following anti‑CD3 stimulation. The stimulation of blood antigen‑presenting cells by lipopolysaccharide, however, did not improve tumor T‑cell proliferation. Overall, the present results provided a viable strategy for improving tumor‑infiltrating CD3+ T‑cell responses in GBM patients.
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Copy and paste a formatted citation
Spandidos Publications style
Han S, Ma E, Wang X, Yu C, Dong T, Zhan W, Wei X, Liang G and Feng S: Rescuing defective tumor‑infiltrating T‑cell proliferation in glioblastoma patients. Oncol Lett 12: 2924-2929, 2016.
APA
Han, S., Ma, E., Wang, X., Yu, C., Dong, T., Zhan, W. ... Feng, S. (2016). Rescuing defective tumor‑infiltrating T‑cell proliferation in glioblastoma patients. Oncology Letters, 12, 2924-2929. https://doi.org/10.3892/ol.2016.4944
MLA
Han, S., Ma, E., Wang, X., Yu, C., Dong, T., Zhan, W., Wei, X., Liang, G., Feng, S."Rescuing defective tumor‑infiltrating T‑cell proliferation in glioblastoma patients". Oncology Letters 12.4 (2016): 2924-2929.
Chicago
Han, S., Ma, E., Wang, X., Yu, C., Dong, T., Zhan, W., Wei, X., Liang, G., Feng, S."Rescuing defective tumor‑infiltrating T‑cell proliferation in glioblastoma patients". Oncology Letters 12, no. 4 (2016): 2924-2929. https://doi.org/10.3892/ol.2016.4944
Copy and paste a formatted citation
x
Spandidos Publications style
Han S, Ma E, Wang X, Yu C, Dong T, Zhan W, Wei X, Liang G and Feng S: Rescuing defective tumor‑infiltrating T‑cell proliferation in glioblastoma patients. Oncol Lett 12: 2924-2929, 2016.
APA
Han, S., Ma, E., Wang, X., Yu, C., Dong, T., Zhan, W. ... Feng, S. (2016). Rescuing defective tumor‑infiltrating T‑cell proliferation in glioblastoma patients. Oncology Letters, 12, 2924-2929. https://doi.org/10.3892/ol.2016.4944
MLA
Han, S., Ma, E., Wang, X., Yu, C., Dong, T., Zhan, W., Wei, X., Liang, G., Feng, S."Rescuing defective tumor‑infiltrating T‑cell proliferation in glioblastoma patients". Oncology Letters 12.4 (2016): 2924-2929.
Chicago
Han, S., Ma, E., Wang, X., Yu, C., Dong, T., Zhan, W., Wei, X., Liang, G., Feng, S."Rescuing defective tumor‑infiltrating T‑cell proliferation in glioblastoma patients". Oncology Letters 12, no. 4 (2016): 2924-2929. https://doi.org/10.3892/ol.2016.4944
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