Leucine zipper, down regulated in cancer‑1 gene expression in prostate cancer

Salemi,Michele; Barone,Nunziata; La Vignera,Sandro; Condorelli,Rosita A.; Recupero,Domenico; Galia,Antonio; Fraggetta,Filippo; Aiello,Anna Maria; Pepe,Pietro; Castiglione,Roberto; Vicari,Enzo; Calogero,Aldo E.

doi:10.3892/ol.2016.4983

Leucine zipper, down regulated in cancer‑1 gene expression in prostate cancer

Authors:
- Michele Salemi
- Nunziata Barone
- Sandro La Vignera
- Rosita A. Condorelli
- Domenico Recupero
- Antonio Galia
- Filippo Fraggetta
- Anna Maria Aiello
- Pietro Pepe
- Roberto Castiglione
- Enzo Vicari
- Aldo E. Calogero
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Affiliations: Laboratory of Cytogenetics, National Institute for Research and Treatment Oasi Maria SS, Institute for Research on Mental Retardation and Brain Aging, I-94018 Troina, Italy, Department of Clinical and Experimental Medicine, Section of Endocrinology, Andrology and Internal Medicine, University of Catania, I-95123 Catania, Italy, Pathology Unit, Cannizzaro Hospital, I-95100 Catania, Italy, Urology Unit, Cannizzaro Hospital, I-95100 Catania, Italy
Published online on: August 10, 2016 https://doi.org/10.3892/ol.2016.4983
Pages: 2796-2800

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Abstract

Numerous genetic alterations have been implicated in the development of prostate cancer (PCa). DNA and protein microarrays have enabled the identification of genes associated with apoptosis, which is important in PCa development. Despite the molecular mechanisms are not entirely understood, inhibition of apoptosis is a critical pathophysiological factor that contributes to the onset and progression of PCa. Leucine zipper, down‑regulated in cancer 1 (LDOC‑1) is a known regulator of the nuclear factor (NF)‑mediated pathway of apoptosis through the inhibition of NF‑κB. The present study investigated the expression of the LDOC‑1 gene in LNCaP, PC‑3, PNT1A and PNT2 prostate cell lines by reverse transcription‑quantitative polymerase chain reaction. In addition LDOC‑1 protein expression in normal prostate tissues and PCa was studied by immunohistochemistry. LDOC‑1 messenger RNA resulted overexpressed in LNCaP and PC‑3 PCa cell lines compared with the two normal prostate cell lines PNT1A and PNT2. The results of immunohistochemistry demonstrated a positive cytoplasmic LDOC‑1 staining in all PCa and normal prostate samples, whereas no nuclear staining was observed in any sample. Furthermore, a more intense signal was evidenced in PCa samples. LDOC‑1 gene overexpression in PCa suggests an activity of LDOC‑1 in PCa cell lines.

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