Iodine‑125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA‑181c expression

  • Authors:
    • Yong Yang
    • Zhen‑Huan Ma
    • Xiao‑Gang Li
    • Wan‑Fu Zhang
    • Jia Wan
    • Ling‑Juan Du
    • Guo‑Jian Li
    • Guo‑Kai Yang
    • Ping Lu
  • View Affiliations

  • Published online on: August 17, 2016     https://doi.org/10.3892/ol.2016.5033
  • Pages: 2789-2795
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Abstract

Iodine-125 (125I) seed implantation has been widely used for the treatment of unresectable advanced tumors. However, the molecular mechanisms underlying the tumor‑suppressive effects of 125I irradiation have not been fully elucidated. The present study demonstrated that 125I irradiation suppresses cell viability and inhibits cell invasiveness of gastric cancer KATO‑III and MKN45 cells. Further mechanistic analysis suggested the involvement of microRNA (miR)‑181c in the inhibitory effects induced by 125I irradiation. Methylated DNA immunoprecipitation coupled with quantitative‑polymerase chain reaction demonstrated that treatment with 125I irradiation, at the dose of 4 Gy, induced promoter demethylation of the miR‑181c gene in KATO‑III and MKN45 cells. Following irradiation, the expression of miR‑181c was significantly increased, which may be attributed to the demethylation caused by 125I irradiation. In addition, upregulation of miR‑181c by administration of miR‑181c mimics decreased cell invasion, suggesting the role of miR‑181c as a tumor suppressor. More importantly, the tumor‑suppressive effects of 125I irradiation were significantly compromised by the introduction of miR‑181c inhibitors. Overall, these results reveal that 125I irradiation inhibits invasiveness of gastric cancer cells by reactivating miR‑181c at the epigenetic level, thereby providing important molecular evidence for the anticancer effects of 125I irradiation.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yang Y, Ma ZH, Li XG, Zhang WF, Wan J, Du LJ, Li GJ, Yang GK and Lu P: Iodine‑125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA‑181c expression. Oncol Lett 12: 2789-2795, 2016.
APA
Yang, Y., Ma, Z., Li, X., Zhang, W., Wan, J., Du, L. ... Lu, P. (2016). Iodine‑125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA‑181c expression. Oncology Letters, 12, 2789-2795. https://doi.org/10.3892/ol.2016.5033
MLA
Yang, Y., Ma, Z., Li, X., Zhang, W., Wan, J., Du, L., Li, G., Yang, G., Lu, P."Iodine‑125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA‑181c expression". Oncology Letters 12.4 (2016): 2789-2795.
Chicago
Yang, Y., Ma, Z., Li, X., Zhang, W., Wan, J., Du, L., Li, G., Yang, G., Lu, P."Iodine‑125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA‑181c expression". Oncology Letters 12, no. 4 (2016): 2789-2795. https://doi.org/10.3892/ol.2016.5033