Effect of matrine combined with cisplatin on the expression of XIAP in human rhabdomyosarcoma RD cells

Li,Li; Xue,Tianyang; Xu,Wei; Zhou,Bin

doi:10.3892/ol.2016.5150

Effect of matrine combined with cisplatin on the expression of XIAP in human rhabdomyosarcoma RD cells

Authors:
- Li Li
- Tianyang Xue
- Wei Xu
- Bin Zhou
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Affiliations: Department of Pediatrics, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China, Pediatric Hospital of The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221000, P.R. China
Published online on: September 19, 2016 https://doi.org/10.3892/ol.2016.5150
Pages: 3793-3798
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The combined effects of matrine (Mat) and cisplatin on the survival and apoptosis of rhabdomyosarcoma (RMS) RD cells, as well as the possible mechanism of the synergistic effect of Mat and cisplatin were investigated in the present study. RMS RD cells were divided and treated as follows: control group, 5 mg/l cisplatin group, Mat groups (0.5, 1.0 and 1.5 g/l), and Mat (0.5, 1.0 and 1.5 g/l) combined with 5 mg/l cisplatin groups. An MTT assay and flow cytometry were applied to detect the survival and apoptotic rates, respectively, while RT-PCR was applied to detect the expression levels of X-linked inhibitor of apoptosis protein (XIAP) mRNA in the RD cells of each group. The survival rates of RD cells in each experimental group were lower than in the control group, and the apoptotic rates were higher than those in the control group (P<0.05). An increase in drug concentrations led to the cell proliferation inhibitory and apoptotic rates of the single Mat groups increasing as a function of dose (pairwise comparison among the groups, P<0.05), while the proliferation inhibitory and apoptotic rates of Mat combined with the cisplatin groups under different concentration were significantly higher than those of the single Mat and single cisplatin groups under the same concentration (P<0.01). The expression levels of XIAP mRNA in the RD cells of each experimental group were lower than those in the control group (P<0.05). Additionally, the expression levels of XIAP mRNA in the group treated with Mat and cisplatin were significantly lower than those of the single cisplatin and single Mat groups (P<0.01). In conclusion, Mat and cisplatin are capable of inhibiting the proliferation of RD cells and inducing apoptosis by suppressing the XIAP mRNA expression levels.

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1	Song YQ, Liu SP, Liu ZF and Hu XL: Determination of matrine and oxymatrine in Radix Sophorae Flavescentis by resonance rayleigh scattering, second-order scattering and frequency doubling scattering technique. Chem Res Chin Univ. 27:746–749. 2011.
2	Zhaowu Z, Xiaoli W, Yangde Z and Nianfeng L: Preparation of matrine ethosome, its percutaneous permeation in vitro and anti-inflammatory activity in vivo in rats. J Liposome Res. 19:155–162. 2009. View Article : Google Scholar : PubMed/NCBI
3	Niu H, Zhang Y, Wu B, Zhang Y, Jiang H and He P: Matrine induces the apoptosis of lung cancer cells through downregulation of inhibitor of apoptosis proteins and the Akt signaling pathway. Oncol Rep. 32:1087–1093. 2014.PubMed/NCBI
4	Liu YQ, Li Y, Qin J, Wang Q, She YL, Luo YL, He JX, Li JY and Xie XD: Matrine reduces proliferation of human lung cancer cells by inducing apoptosis and changing miRNA expression profiles. Asian Pac J Cancer Prev. 15:2169–2177. 2014. View Article : Google Scholar : PubMed/NCBI
5	Li H, Li X, Bai M, Suo Y, Zhang G and Cao X: Matrine inhibited proliferation and increased apoptosis in human breast cancer MCF-7 cells via upregulation of Bax and downregulation of Bcl-2. Int J Clin Exp Pathol. 8:14793–14799. 2015.PubMed/NCBI
6	Gao H, Guo Y, Deng N, Fei P, Qiu X, Zheng P, Feng J and Dai G: Suppressive effect of matrine on tumor invasion in N-butyl-N-4-hydroxybutyl)nitrosamine-induced urinary bladder carcinogenesis. Chemotherapy. 60:119–128. 2014. View Article : Google Scholar : PubMed/NCBI
7	Li Q, Lai Y, Wang C, Xu G, He Z, Shang X, Sun Y, Zhang F, Liu L and Huang H: Matrine inhibits the proliferation, invasion and migration of castration-resistant prostate cancer cells through regulation of the NF-κB signaling pathway. Oncol Rep. 35:375–381. 2016.PubMed/NCBI
8	Rong B, Zhao C, Gao W and Yang S: Matrine promotes the efficacy and safety of platinum-based doublet chemotherapy for advanced non-small cell lung cancer. Int J Clin Exp Med. 8:14701–14717. 2015.PubMed/NCBI
9	Wang HQ, Jin JJ and Wang J: Matrine induces mitochondrial apoptosis in cisplatin-resistant non-small cell lung cancer cells via suppression of β-catenin/survivin signaling. Oncol Rep. 33:2561–2566. 2015.PubMed/NCBI
10	Flanagan L, Sebastià J, Tuffy LP, Spring A, Lichawska A, Devocelle M, Prehn JH and Rehm M: XIAP impairs Smac release from the mitochondria during apoptosis. Cell Death Dis. 1:e492010. View Article : Google Scholar : PubMed/NCBI
11	Kempkensteffen C, Jäger T, Bub J, Weikert S, Hinz S, Christoph F, Krause H, Schostak M, Miller K and Schrader M: The equilibrium of XIAP and Smac/DIABLO expression is gradually deranged during the development and progression of testicular germ cell tumours. Int J Androl. 30:476–483. 2007. View Article : Google Scholar : PubMed/NCBI
12	Ramp U, Krieg T, Caliskan E, Mahotka C, Ebert T, Willers R, Gabbert HE and Gerharz CD: XIAP expression is an independent prognostic marker in clear-cell renal carcinomas. Hum Pathol. 35:1022–1028. 2004. View Article : Google Scholar : PubMed/NCBI
13	Hanson AJ, Wallace HA, Freeman TJ, Beauchamp RD, Lee LA and Lee E: XIAP monoubiquitylates Groucho/TLE to promote canonical Wnt signaling. Mol Cell. 45:619–628. 2012. View Article : Google Scholar : PubMed/NCBI
14	Zheng ZL, Tan LZ, Yu YP, Michalopoulos G and Luo JH: Interaction of CSR1 with XIAP reverses inhibition of caspases and accelerates cell death. Am J Pathol. 181:463–471. 2012. View Article : Google Scholar : PubMed/NCBI
15	Berezovskaya O, Schimmer AD, Glinskii AB, Pinilla C, Hoffman RM, Reed JC and Glinsky GV: Increased expression of apoptosis inhibitor protein XIAP contributes to anoikis resistance of circulating human prostate cancer metastasis precursor cells. Cancer Res. 65:2378–2386. 2005. View Article : Google Scholar : PubMed/NCBI
16	Doudican NA, Byron SA, Pollock PM and Orlow SJ: XIAP downregulation accompanies mebendazole growth inhibition in melanoma xenografts. Anticancer Drugs. 24:181–188. 2013. View Article : Google Scholar : PubMed/NCBI
17	Castells M, Milhas D, Gandy C, Thibault B, Rafii A, Delord JP and Couderc B: Microenvironment mesenchymal cells protect ovarian cancer cell lines from apoptosis by inhibiting XIAP inactivation. Cell Death Dis. 4:e8872013. View Article : Google Scholar : PubMed/NCBI
18	Cao Z, Li X, Li J, Luo W, Huang C and Chen J: X-linked inhibitor of apoptosis protein (XIAP) lacking RING domain localizes to the nuclear and promotes cancer cell anchorage-independent growth by targeting the E2F1/Cyclin E axis. Oncotarget. 5:7126–7137. 2014. View Article : Google Scholar : PubMed/NCBI
19	Liew JC, Tan WS, Alitheen NB, Chan ES and Tey BT: Over-expression of the X-linked inhibitor of apoptosis protein (XIAP) delays serum deprivation-induced apoptosis in CHO-K1 cells. J Biosci Bioeng. 110:338–344. 2010. View Article : Google Scholar : PubMed/NCBI
20	Katragadda L, Carter BZ and Borthakur G: XIAP antisense therapy with AEG 35156 in acute myeloid leukemia. Expert Opin Investig Drugs. 22:663–670. 2013. View Article : Google Scholar : PubMed/NCBI
21	Yang CL, Liu SS, Ma YG, Liu YY, Xue YX and Huang B: The influence of intraoperative pleural perfusion with matrine- cisplatin or cisplatin on stromal cell-derived factor-1 in non-small cell lung cancer patients with subclinical pleural metastasis. Med Oncol. 29:574–581. 2012. View Article : Google Scholar : PubMed/NCBI
22	Rossi S, Stoppani E, Puri PL and Fanzani A: Differentiation of human rhabdomyosarcoma RD cells is regulated by reciprocal, functional interactions between myostatin, p38 and extracellular regulated kinase signalling pathways. Eur J Cancer. 47:1095–1105. 2011. View Article : Google Scholar : PubMed/NCBI
23	Paulino AC and Okcu MF: Rhabdomyosarcoma. Curr Probl Cancer. 32:7–34. 2008. View Article : Google Scholar : PubMed/NCBI
24	Panta P and Felix D: Rhabdomyosarcoma: a rapidly growing malignancy. Pan Afr Med J. 22:1212015. View Article : Google Scholar : PubMed/NCBI
25	Li H, Xie S, Liu X, Wu H, Lin X, Gu J, Wang H and Duan Y: Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells. Oncol Rep. 32:2118–2126. 2014.PubMed/NCBI
26	Meng F, Zhang ZX, Xie J, Huang CB, Liu Y and Liao YG: Effects of matrine on the apoptosis and the expression of PEG10 in human hepatocarcinoma cell Line HepG2. J Pract Med. 10:1523–1526. 2014.(In Chinese).
27	Gao XH, Xu W, Li WJ, Ma HW and Bai YL: Anti-proliferative effects of matrine on human neuroblastoma SK-N-SH cells Chin. J Exp Trad Med Formulae. 23:196–199. 2013.(In Chinese).
28	Yu Q, Chen B, Zhang X, Qian W, Ye B and Zhou Y: Arsenic trioxide-enhanced, matrine-induced apoptosis in multiple myeloma cell lines. Planta Med. 79:775–781. 2013. View Article : Google Scholar : PubMed/NCBI
29	Yan F, Liu Y and Wang W: Matrine inhibited the growth of rat osteosarcoma UMR-108 cells by inducing apoptosis in a mitochondrial-caspase-dependent pathway. Tumour Biol. 34:2135–2140. 2013. View Article : Google Scholar : PubMed/NCBI
30	Guo L, Xue TY, Xu W and Gao JZ: Matrine promotes G0/G1 arrest and down-regulates cyclin D1 expression in human rhabdomyosarcoma cells. Panminerva Med. 55:291–296. 2013.PubMed/NCBI
31	Tan C, Qian X, Jia R, Wu M and Liang Z: Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells. Oncol Rep. 30:2529–2535. 2013.PubMed/NCBI
32	Liu Y, Xu Y, Ji W, Li X, Sun B, Gao Q and Su C: Anti-tumor activities of matrine and oxymatrine: literature review. Tumour Biol. 35:5111–5119. 2014. View Article : Google Scholar : PubMed/NCBI
33	Guo L, Xue TY, Xu W and Gao JZ: Effects of matrine on the proliferation and apoptosis of human rhabdomyosarcoma RD cells. Zhongguo Dang Dai Er Ke Za Zhi. 14:780–784. 2012.(In Chinese). PubMed/NCBI
34	Li YX: Effects and mechanisms of matrine on proliferation and apoptosis of human rhabdomyosarcoma cell RD. Seek Med Ask Med:. 10:295–296. 2012.
35	Miao L, Guo S, Zhang J, Kim WY and Huang L: Nanoparticles with precise ratiometric co-loading and co-delivery of gemcitabine monophosphate and cisplatin for treatment of bladder cancer. Adv Funct Mater. 24:6601–6611. 2014. View Article : Google Scholar : PubMed/NCBI
36	Sárosi V and Lénárt T: Gemcitabine-cisplatin combination in the first line treatment of non-small cell lung cancer. Our experience and analysis of safety. Magy Onkol. 47:189–193. 2003.(In Hungarian). PubMed/NCBI
37	Solár P, Horváth V, Kleban J, Koval' J, Solárová Z, Kozubík A and Fedorocko P: Hsp90 inhibitor geldanamycin increases the sensitivity of resistant ovarian adenocarcinoma cell line A2780cis to cisplatin. Neoplasma. 54:127–130. 2007.PubMed/NCBI
38	Huang Q, Lu G, Shen HM, Chung MC and Ong CN: Anti-cancer properties of anthraquinones from rhubarb. Med Res Rev. 27:609–630. 2007. View Article : Google Scholar : PubMed/NCBI
39	Yin PH, Liu X, Qiu YY, Cai JF, Qin JM, Zhu HR and Li Q: Anti-tumor activity and apoptosis-regulation mechanisms of bufalin in various cancers: new hope for cancer patients. Asian Pac J Cancer Prev. 13:5339–5343. 2012. View Article : Google Scholar : PubMed/NCBI
40	Tang MX: Reduction of metastasis of lung cancer by ‘Fufang Shengmai-compound pulse inducer’ - a preliminary report on experimental, clinical and pathologic study with reference to the ultrastructure. Zhonghua Zhong Liu Za Zhi. 5:176–179. 1983.(In Chinese). PubMed/NCBI
41	Xiao WL, Motley TJ, Unachukwu UJ, Lau CB, Jiang B, Hong F, Leung PC, Wang QF, Livingston PO, Cassileth BR, et al: Chemical and genetic assessment of variability in commercial Radix Astragali (Astragalus spp.) by ion trap LC-MS and nuclear ribosomal DNA barcoding sequence analyses. J Agric Food Chem. 59:1548–1556. 2011. View Article : Google Scholar : PubMed/NCBI
42	Amantana A, London CA, Iversen PL and Devi GR: X-linked inhibitor of apoptosis protein inhibition induces apoptosis and enhances chemotherapy sensitivity in human prostate cancer cells. Mol Cancer Ther. 3:699–707. 2004.PubMed/NCBI