Open Access

Gene expression profiling of the 8q22-24 position in human breast cancer: TSPYL5, MTDH, ATAD2 and CCNE2 genes are implicated in oncogenesis, while WISP1 and EXT1 genes may predict a risk of metastasis

  • Authors:
    • Afsoon Taghavi
    • Mohammad Esmaeil Akbari
    • Mohammad Hashemi‑Bahremani
    • Nahid Nafissi
    • Ahad Khalilnezhad
    • Seyed Mohammad Poorhosseini
    • Feyzollah Hashemi‑Gorji
    • Vahid Reza Yassaee
  • View Affiliations

  • Published online on: September 30, 2016     https://doi.org/10.3892/ol.2016.5218
  • Pages: 3845-3855
  • Copyright: © Taghavi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gene expression profiling has been suggested to predict breast cancer outcome. The prognostic value of the 8q22‑24 position in breast cancer remains to be elucidated. The present study evaluated expression patterns of the genes located at this position in metastatic and non‑metastatic breast cancer. A total of 85 patients with recurrent/metastatic (n=15) and non‑metastatic (n=70) early‑stage, estrogen receptor‑positive and lymph node‑negative breast tumors were included. In addition, 15 normal breast tissue samples were used as controls. Demographic and clinical features were recorded. Subsequently, mRNA copy numbers of exostosin glycosyltransferase 1 (EXT1), WNT1 inducible signaling pathway protein 1 (WISP1), ATPase family, AAA domain containing 2 (ATAD2), TSP‑like 5 (TSPYL5), metadherin (MTDH) and cyclin E2 (CCNE2) genes were measured by reverse transcription‑quantitative polymerase chain reaction assay. The expression of EXT1 and WISP1 exhibited a significant decline in the metastatic breast cancer group compared to the control (P=0.015 and P=0.012, respectively). The expression of TSPYL5, MTDH and ATAD2 was significantly decreased in the metastatic (P=0.002, P=0.018 and P=0.016, respectively) and non‑metastatic (P=0.038, P=0.045 and P=0.000, respectively) breast cancer groups compared with the control. The expression of CCNE2 in the metastatic and non‑metastatic breast cancer groups was significantly increased compared with the control (P=0.002 and P=0.001, respectively). WISP1 expression demonstrated a correlation with patient age and tumor size, and TSPYL5 expression was correlated with lymphovascular invasion. None of the genes investigated exhibited any correlation with stage and grade of disease. The TSPYL5, MTDH, ATAD2 and CCNE2 genes may be implicated in the pathogenesis of human breast cancer, while the WISP1 and EXT1 genes may have the potential to serve as promising indicators of the risk of metastasis. However, further studies are required to validate these results.
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November-2016
Volume 12 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Taghavi A, Akbari ME, Hashemi‑Bahremani M, Nafissi N, Khalilnezhad A, Poorhosseini SM, Hashemi‑Gorji F and Yassaee VR: Gene expression profiling of the 8q22-24 position in human breast cancer: TSPYL5, MTDH, ATAD2 and CCNE2 genes are implicated in oncogenesis, while WISP1 and EXT1 genes may predict a risk of metastasis. Oncol Lett 12: 3845-3855, 2016.
APA
Taghavi, A., Akbari, M.E., Hashemi‑Bahremani, M., Nafissi, N., Khalilnezhad, A., Poorhosseini, S.M. ... Yassaee, V.R. (2016). Gene expression profiling of the 8q22-24 position in human breast cancer: TSPYL5, MTDH, ATAD2 and CCNE2 genes are implicated in oncogenesis, while WISP1 and EXT1 genes may predict a risk of metastasis. Oncology Letters, 12, 3845-3855. https://doi.org/10.3892/ol.2016.5218
MLA
Taghavi, A., Akbari, M. E., Hashemi‑Bahremani, M., Nafissi, N., Khalilnezhad, A., Poorhosseini, S. M., Hashemi‑Gorji, F., Yassaee, V. R."Gene expression profiling of the 8q22-24 position in human breast cancer: TSPYL5, MTDH, ATAD2 and CCNE2 genes are implicated in oncogenesis, while WISP1 and EXT1 genes may predict a risk of metastasis". Oncology Letters 12.5 (2016): 3845-3855.
Chicago
Taghavi, A., Akbari, M. E., Hashemi‑Bahremani, M., Nafissi, N., Khalilnezhad, A., Poorhosseini, S. M., Hashemi‑Gorji, F., Yassaee, V. R."Gene expression profiling of the 8q22-24 position in human breast cancer: TSPYL5, MTDH, ATAD2 and CCNE2 genes are implicated in oncogenesis, while WISP1 and EXT1 genes may predict a risk of metastasis". Oncology Letters 12, no. 5 (2016): 3845-3855. https://doi.org/10.3892/ol.2016.5218