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Article Open Access

Time-dependent transition of the immunoglobulin G subclass and immunoglobulin E response in cancer patients vaccinated with cholesteryl pullulan-melanoma antigen gene-A4 nanogel

  • Authors:
    • Noriaki Kyogoku
    • Hiroaki Ikeda
    • Takahiro Tsuchikawa
    • Takehiro Abiko
    • Aki Fujiwara
    • Takehiro Maki
    • Yoshiyuki Yamamura
    • Masaomi Ichinokawa
    • Kimitaka Tanaka
    • Naoko Imai
    • Yoshihiro Miyahara
    • Shinichi Kageyama
    • Hiroshi Shiku
    • Satoshi Hirano
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterological Surgery II, Division of Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060‑8638, Japan, Department of Immuno‑Gene Therapy, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan
    Copyright: © Kyogoku et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4493-4504
    |
    Published online on: October 13, 2016
       https://doi.org/10.3892/ol.2016.5253
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Abstract

A phase I+II clinical trial of vaccination with MAGE‑A4 protein complexed with cholesteryl pullulan melanoma antigen gene-A4 nanogel (CHP-MAGE-A4) is currently underway in patients with MAGE‑A4‑expressing cancer. In the present study, the primary phase I endpoint was to test the safety of the administration of 300 µg CHP‑MAGE‑A4 with and without OK‑432. Another aim of the study was to clarify the details of the specific humoral immune response to vaccination. The 9 patients enrolled for phase I were vaccinated 6 times, once every 2 weeks: 3 patients with 100 µg and 3 patients with 300 µg CHP‑MAGE‑A4, and 3 patients with 300 µg CHP‑MAGE‑A4 plus 0.5 clinical units of OK‑432. Toxicities were assessed using Common Terminology Criteria for Adverse Events v3.0. Clinical response was evaluated by modified Response Evaluation Criteria in Solid Tumours. Immunological monitoring of anti‑MAGE‑A4‑specific antibodies was performed by ELISA of pre‑ and post‑vaccination patient sera. The 6 vaccinations produced no severe adverse events. Stable disease was assessed in 4/9 patients. Anti‑MAGE‑A4 total immunoglobulin (Ig)G titers increased in 7/9 patients. Efficacious anti‑MAGE‑A4 IgG1, 2 and 3 antibody responses were observed in 7/9 patients. Among them, positive conversions to T helper 2 (Th2)‑type antibody responses (IgG4 and IgE) were observed after frequent vaccination in 4/7 patients. The Th2 conversion was possibly associated with undesirable clinical observations, including progressive disease and the appearance of a new relapse lesion. The present study suggested that frequent vaccinations activated a Th2‑dominant status in the cancer patients. The identification of a time‑dependent IgG subclass and IgE antibody production during vaccination protocols may be a useful surrogate marker indicating a potentially undesirable change of the immunological environment for an effective antitumor immune response in cancer patients.
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1 

Rosenberg SA, Yang JC and Restifo NP: Cancer immunotherapy: Moving beyond current vaccines. Nat Med. 10:909–915. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Itoh K, Yamada A, Mine T and Noguchi M: Recent advances in cancer vaccines: An overview. Jpn J Clin Oncol. 39:73–80. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Mine T, Sato Y, Noguchi M, Sasatomi T, Gouhara R, Tsuda N, Tanaka S, Shomura H, Katagiri K, Rikimaru T, et al: Humoral responses to peptides correlate with overall survival in advanced cancer patients vaccinated with peptides based on pre-existing, peptide-specific cellular responses. Clin Cancer Res. 10:929–937. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Ullenhag GJ, Frödin JE, Strigård K, Mellstedt H and Magnusson CG: Induction of IgG subclass responses in colorectal carcinoma patients vaccinated with recombinant carcinoembryonic antigen. Cancer Res. 62:1364–1369. 2002.PubMed/NCBI

5 

Aoki M, Ueda S, Nishikawa H, Kitano S, HIrayama M, Ikeda H, Toyoda H, Tanaka K, Kanai M, Takabayashi A, et al: Antibody responses against NY-ESO-1 and HER2 antigens in patients vaccinated with combinations of cholesteryl pullulan (CHP)-NY-ESO-1 and CHP-HER2 with OK-432. Vaccine. 27:6854–6861. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Kageyama S, Kitano S, Hirayama M, Nagata Y, Imai H, Shiraishi T, Akiyoshi K, Scott AM, Murphy R, Hoffman EW, et al: Humoral immune responses in patients vaccinated with 1–146 HER2 protein complexed with cholesteryl pullulan nanogel. Cancer Sci. 99:601–607. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Karagiannis P, Gilbert AE, Josephs DH, Ali N, Dodev T, Saul L, Correa I, Roberts L, Beddowes E, Koers A, et al: IgG4 subclass antibodies impair antitumor immunity in melanoma. J Clin Invest. 123:1457–1474. 2013. View Article : Google Scholar : PubMed/NCBI

8 

De Plaen E, Naerhuyzen B, De Smet C, Szikora JP and Boon T: Alternative promoters of gene MAGE4a. Genomics. 40:305–313. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Scanlan MJ, Gure AO, Jungbluth AA, Old LJ and Chen YT: Cancer/testis antigens: An expanding family of targets for cancer immunotherapy. Immuno Rev. 188:22–32. 2002. View Article : Google Scholar

10 

Resnick MB, Sabo E, Kondratev S, Kerner H, Spagnoli GC and Yakirevich E: Cancer-testis antigen expression in uterine malignancies with an emphasis on carcinosarcomas and papillary serous carcinomas. Int J Cancer. 101:190–195. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Yakirevich E, Sabo E, Lavie O, Mazareb S, Spagnoli GC and Resnick MB: Expression of the MAGE-A4 and NY-ESO-1 cancer-testis antigens in serous ovarian neoplasms. Clin Can Res. 9:6453–6460. 2003.

12 

Tajima K, Obata Y, Tamaki H, Tamaki H, Yoshida M, Chen YT, Scanlan MJ, Old LJ, Kuwano H, Takahashi T, et al: Expression of cancer/testis (CT) antigens in lung cancer. Lung Cancer. 42:23–33. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Prasad ML, Jungbluth AA, Petal SG, Iversen K, Hoshaw-Woodard S and Busam KJ: Expression and significance of cancer/testis antigens in primary mucosal melanoma of the head and neck. Head Neck. 26:1053–1057. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Li M, Yuan YH, Han Y, Liu YX, Yan L, Wang Y and Gu J: Expression profile of cancer-testis genes in 121 human colorectal cancer tissue and adjacent normal tissue. Clin Can Res. 11:1809–1814. 2005. View Article : Google Scholar

15 

Gure AO, Chua R, Williamson B, Gonen M, Ferrera CA, Gnjatic S, Ritter G, Simpson AJ, Chen YT, Old LJ and Altorki NK: Cancer-testis genes are coordinately expressed and are markers of poor outcome in non-small cell lung cancer. Clin Can Res. 11:8055–8062. 2005. View Article : Google Scholar

16 

Peng JR, Chen HS, Mou DC, Cao J, Cong X, Qin LL, Wei L, Leng XS, Wang Y and Chen WF: Expression of cancer/testis (CT) antigens in Chinese hepatocellular carcinoma and its correlation with clinical parameters. Cancer Lett. 219:223–232. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Barrow C, Browning J, MacGregor D, Davis ID, Sturrock S, Jungbluth AA and Cebon J: Tumor antigen expression in melanoma varies according to antigen and stage. Clin Can Res. 12:764–771. 2006. View Article : Google Scholar

18 

Akcakanat A, Kanda T, Tanabe T, Komukai S, Yajima K, Nakagawa S, Ohashi M and Hatakeyama K: Heterogeneous expression of GAGE, NY-ESO-1, MAGE-A and SSX proteins in esophageal cancer: Implications for immunotherapy. Int J Cancer. 118:123–128. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Kageyama S, Wada H, Muro K, Niwa Y, Ueda S, Miyata H, Takiguchi S, Sugino SH, Miyahara Y, Ikeda H, et al: Dose-dependent effects of NY-ESO-1 protein vaccine complexed with cholesteryl pullulan (CHP-NY-ESO-1) on immune responses and survival benefits of esophageal cancer patients. J Transl Med. 11:2462013. View Article : Google Scholar : PubMed/NCBI

20 

Gu XG, Schmitt M, Hiasa A, Nagata Y, Ikeda H, Sasaki Y, Akiyoshi K, Sunamoto J, Nakamura H, Kuribayashi K and Shiku H: A novel hydrophobized polysaccharide/oncoprotein complex vaccine induces in vitro and in vivo cellular and humoral immune responses against HER2-expressing murine sarcomas. Cancer Res. 58:3385–3390. 1998.PubMed/NCBI

21 

Okamoto M, Furuichi S, Nishioka Y, Oshikawa T, Tano T, Ahmed SU, Takeda K, Akira S, Ryoma Y, Moriya Y, et al: Expression of toll-like receptor 4 on dendritic cells is significant for anticancer effect of dendritic cell-based immunotherapy in combination with an active component of OK-432, a streptococcal preparation. Cancer Res. 64:5461–5470. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Nakahara S, Tsunoda T, Baba T, Asabe S and Tahara H: Dendritic cells stimulated with a bacterial product, OK-432, efficiently induce cytotoxic T lymphocytes specific to tumor rejection peptide. Cancer Res. 63:4112–4118. 2003.PubMed/NCBI

23 

No authors listed, . Japanese translation of common terminology criteria for adverse events (CTCAE), and instructions and guidelines. Int J Clin Oncol. 9:(Suppl 3). 1–82. 2004.(In Japanese).

24 

Wolchok JD, Hoos A, O'Day S, Weber JS, Hamid O, Lebbe C, Maio M, Binder M, Bohnsack O, Nichol G, et al: Guidelines for the evaluation of immune therapy activity in solid tumors: Immune-related response criteria. Clin Cancer Res. 15:7412–7420. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Oken MM, Creech RH, Tormey DC, Horton J, Davis TE, McFadden ET and Carbone PP: Toxicity and response criteria of the Eastern Cooperative Oncology Group. Am J Clin Oncol. 5:649–655. 1982. View Article : Google Scholar : PubMed/NCBI

26 

Nigro EA, Brini AT, Soprana E, Ambrosi A, Dombrowicz D, Siccardi AG and Vangelista L: Antitumor IgE adjuvanticity: Key role of Fc epsilon RI. J Immunol. 183:4530–4536. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Reali E, Greiner JW, Corti A, Gould HJ, Bottazzoli F, Paganelli G, Schlom J and Siccardi AG: IgEs targeted on tumor cells: Therapeutic activity and potential in the design of tumor vaccines. Cancer Res. 61:5517–5522. 2001.PubMed/NCBI

28 

Yoshida K, Noguchi M, Mine T, Komatu N, Yutani S, Ueno T, Yanagimoto H, Kawano K, Itoh K and Yamada A: Characteristics of severe adverse events after peptide vaccination for advanced cancer patients: Analysis of 500 cases. Oncol Rep. 25:57–62. 2011.PubMed/NCBI

29 

Kidd P: Th1/Th2 balance: The hypothesis, its limitations, and implications for health and disease. Altern Med Rev. 8:223–246. 2003.PubMed/NCBI

30 

Romagnani S: Th1 and Th2 in human diseases. Clin Immunol Immunopathol. 80:225–235. 1996. View Article : Google Scholar : PubMed/NCBI

31 

Shaw DR, Khazaeli MB and LoBuglio AF: Mouse/human chimeric antibodies to a tumor-associated antigen: Biologic activity of the four human IgG subclasses. J Natl Cancer Inst. 80:1553–1559. 1988. View Article : Google Scholar : PubMed/NCBI

32 

Jutel M and Akdis CA: Immunological mechanisms of allergen-specific immunotherapy. Allergy. 66:725–732. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Aalberse RC, Stapel SO, Schuurman J and Rispens T: Immunoglobulin G4: An odd antibody. Clin Exp Allergy. 39:469–477. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Riemer AB, Untersmayr E, Knittelfelder R, Duschl A, Pehamberger H, Zielinski CC, Scheiner O and Jensen-Jarolim E: Active induction of tumor-specific IgE antibodies by oral mimotope vaccination. Cancer Res. 67:3406–3411. 2007. View Article : Google Scholar : PubMed/NCBI

35 

Yamada A, Yano H, Takao Y, Ono T, Mastumoto T and Itoh K: Nonmutated self-antigen-derived cancer vaccine peptides elicit an IgE-independent but mast cell-dependent immediate-type skin reaction without systemic anaphylaxis. J Immunol. 176:857–863. 2006. View Article : Google Scholar : PubMed/NCBI

36 

Ohkouchi S, Yamada A, Imai N, Mine T, Harada K, Shichijo S, Maeda Y, Saijo Y, Nukiwa T and Itoh K: Non-mutated tumor-rejection antigen peptides elicit type-I allergy in the majority of healthy individuals. Tissue Antigens. 59:259–272. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kyogoku N, Ikeda H, Tsuchikawa T, Abiko T, Fujiwara A, Maki T, Yamamura Y, Ichinokawa M, Tanaka K, Imai N, Imai N, et al: Time-dependent transition of the immunoglobulin G subclass and immunoglobulin E response in cancer patients vaccinated with cholesteryl pullulan-melanoma antigen gene-A4 nanogel. Oncol Lett 12: 4493-4504, 2016.
APA
Kyogoku, N., Ikeda, H., Tsuchikawa, T., Abiko, T., Fujiwara, A., Maki, T. ... Hirano, S. (2016). Time-dependent transition of the immunoglobulin G subclass and immunoglobulin E response in cancer patients vaccinated with cholesteryl pullulan-melanoma antigen gene-A4 nanogel. Oncology Letters, 12, 4493-4504. https://doi.org/10.3892/ol.2016.5253
MLA
Kyogoku, N., Ikeda, H., Tsuchikawa, T., Abiko, T., Fujiwara, A., Maki, T., Yamamura, Y., Ichinokawa, M., Tanaka, K., Imai, N., Miyahara, Y., Kageyama, S., Shiku, H., Hirano, S."Time-dependent transition of the immunoglobulin G subclass and immunoglobulin E response in cancer patients vaccinated with cholesteryl pullulan-melanoma antigen gene-A4 nanogel". Oncology Letters 12.6 (2016): 4493-4504.
Chicago
Kyogoku, N., Ikeda, H., Tsuchikawa, T., Abiko, T., Fujiwara, A., Maki, T., Yamamura, Y., Ichinokawa, M., Tanaka, K., Imai, N., Miyahara, Y., Kageyama, S., Shiku, H., Hirano, S."Time-dependent transition of the immunoglobulin G subclass and immunoglobulin E response in cancer patients vaccinated with cholesteryl pullulan-melanoma antigen gene-A4 nanogel". Oncology Letters 12, no. 6 (2016): 4493-4504. https://doi.org/10.3892/ol.2016.5253
Copy and paste a formatted citation
x
Spandidos Publications style
Kyogoku N, Ikeda H, Tsuchikawa T, Abiko T, Fujiwara A, Maki T, Yamamura Y, Ichinokawa M, Tanaka K, Imai N, Imai N, et al: Time-dependent transition of the immunoglobulin G subclass and immunoglobulin E response in cancer patients vaccinated with cholesteryl pullulan-melanoma antigen gene-A4 nanogel. Oncol Lett 12: 4493-4504, 2016.
APA
Kyogoku, N., Ikeda, H., Tsuchikawa, T., Abiko, T., Fujiwara, A., Maki, T. ... Hirano, S. (2016). Time-dependent transition of the immunoglobulin G subclass and immunoglobulin E response in cancer patients vaccinated with cholesteryl pullulan-melanoma antigen gene-A4 nanogel. Oncology Letters, 12, 4493-4504. https://doi.org/10.3892/ol.2016.5253
MLA
Kyogoku, N., Ikeda, H., Tsuchikawa, T., Abiko, T., Fujiwara, A., Maki, T., Yamamura, Y., Ichinokawa, M., Tanaka, K., Imai, N., Miyahara, Y., Kageyama, S., Shiku, H., Hirano, S."Time-dependent transition of the immunoglobulin G subclass and immunoglobulin E response in cancer patients vaccinated with cholesteryl pullulan-melanoma antigen gene-A4 nanogel". Oncology Letters 12.6 (2016): 4493-4504.
Chicago
Kyogoku, N., Ikeda, H., Tsuchikawa, T., Abiko, T., Fujiwara, A., Maki, T., Yamamura, Y., Ichinokawa, M., Tanaka, K., Imai, N., Miyahara, Y., Kageyama, S., Shiku, H., Hirano, S."Time-dependent transition of the immunoglobulin G subclass and immunoglobulin E response in cancer patients vaccinated with cholesteryl pullulan-melanoma antigen gene-A4 nanogel". Oncology Letters 12, no. 6 (2016): 4493-4504. https://doi.org/10.3892/ol.2016.5253
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