Detection of aberrant methylated SEPT9 and NTRK3 genes in sporadic colorectal cancer patients as a potential diagnostic biomarker

  • Authors:
    • Shahin Behrouz Sharif
    • Shahriar Hashemzadeh
    • Reza Mousavi Ardehaie
    • Amirtaher Eftekharsadat
    • Mortaza Ghojazadeh
    • Amir Hossein Mehrtash
    • Mehrdad Asghari Estiar
    • Ladan Teimoori‑Toolabi
    • Ebrahim Sakhinia
  • View Affiliations

  • Published online on: October 31, 2016     https://doi.org/10.3892/ol.2016.5327
  • Pages: 5335-5343
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Abstract

Colorectal cancer (CRC) is one of the most common malignancies, and the third leading cause of cancer mortality worldwide. Timely detection of CRC in patients with earlier stages provides the highest rate of survival. Epigenetic alterations are important in the occurrence and progression of CRC, and represent the primary modifications of cancer cells. Therefore, detection of these alterations in CRC cases are thought to hold great promise as diagnostic biomarkers. It has been shown that the SEPT9 and NTRK3 genes are aberrantly methylated and their detection can be used as biomarkers for early diagnosis of CRC. The present study analyzed promoter methylation status of these genes in CRC patients. Genomic DNA was extracted from 45 CRC and paired adjacent healthy tissues and undergone bisulfite conversion, and the methylation status of NTRK3 and SEPT9 were defined using the MS‑HRM assay. Our results showed that there are statistically significant differences in methylation status of NTRK3 and specially SEPT9 between CRC and adjacent normal tissues (P<0.001). High sensitivity and specificity for a specific location in SEPT9 gene promoter as a diagnostic biomarker was observed. SEPT9 promoter hypermethylation may serve as a promising biomarker for the detection of CRC development. However, to validate the biomarker potential of NTRK3 there is a requirement for further investigation.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Behrouz Sharif S , Hashemzadeh S, Mousavi Ardehaie R, Eftekharsadat A, Ghojazadeh M, Mehrtash AH, Estiar MA, Teimoori‑Toolabi L and Sakhinia E: Detection of aberrant methylated SEPT9 and NTRK3 genes in sporadic colorectal cancer patients as a potential diagnostic biomarker. Oncol Lett 12: 5335-5343, 2016.
APA
Behrouz Sharif, S. ., Hashemzadeh, S., Mousavi Ardehaie, R., Eftekharsadat, A., Ghojazadeh, M., Mehrtash, A.H. ... Sakhinia, E. (2016). Detection of aberrant methylated SEPT9 and NTRK3 genes in sporadic colorectal cancer patients as a potential diagnostic biomarker. Oncology Letters, 12, 5335-5343. https://doi.org/10.3892/ol.2016.5327
MLA
Behrouz Sharif, S. ., Hashemzadeh, S., Mousavi Ardehaie, R., Eftekharsadat, A., Ghojazadeh, M., Mehrtash, A. H., Estiar, M. A., Teimoori‑Toolabi, L., Sakhinia, E."Detection of aberrant methylated SEPT9 and NTRK3 genes in sporadic colorectal cancer patients as a potential diagnostic biomarker". Oncology Letters 12.6 (2016): 5335-5343.
Chicago
Behrouz Sharif, S. ., Hashemzadeh, S., Mousavi Ardehaie, R., Eftekharsadat, A., Ghojazadeh, M., Mehrtash, A. H., Estiar, M. A., Teimoori‑Toolabi, L., Sakhinia, E."Detection of aberrant methylated SEPT9 and NTRK3 genes in sporadic colorectal cancer patients as a potential diagnostic biomarker". Oncology Letters 12, no. 6 (2016): 5335-5343. https://doi.org/10.3892/ol.2016.5327