Open Access

MicroRNA-320 regulates the radiosensitivity of cervical cancer cells C33AR by targeting β-catenin

  • Authors:
    • Chun‑Xu Yang
    • Shi‑Min Zhang
    • Jie Li
    • Bo Yang
    • Wen Ouyang
    • Zi‑Jie Mei
    • Jing Chen
    • Jing Dai
    • Su Ke
    • Fu‑Xiang Zhou
    • Yun‑Feng Zhou
    • Cong‑Hua Xie
  • View Affiliations

  • Published online on: November 2, 2016     https://doi.org/10.3892/ol.2016.5340
  • Pages: 4983-4990
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cervical cancer is the second most common malignancy in women worldwide and always has recurrence owing to radioresistance. MicroRNA (miRNA or miR) has been identified to relate to the sensitivity of cancer radiotherapy. Here, we investigated the potential of miRNA‑320 as a biomarker for radiosensitivity by targeting β‑catenin in cervical cancer. A radioresistant cervical cancer cell line, C33AR, was established, and the radioresistance of C33AR cells was confirmed by a colony‑formation assay. The expression of miRNA‑320 was detected by reverse transcription‑quantitative polymerase chain reaction, and compared between C33A and C33AR. β‑catenin, the target of miRNA‑320, was determined at the protein level by western blotting after transfecting the inhibitor of miRNA‑320. The expression of miRNA‑320 was markedly decreased in C33AR cells, which appeared to be more radioresistant, compared with its parental cell line C33A. Target prediction suggested that miRNA‑320 negatively regulated the expression of β‑catenin. Knockdown of β‑catenin increased C33AR radiosensitivity, which revealed that the inhibition of β‑catenin could rescue the miRNA‑320‑mediated cell radioresistance. On the other hand, overexpressing miRNA‑320 increased C33AR radiosensitivity. In conclusion, miRNA‑320 regulated the radiosensitivity of C33AR cells by targeting β‑catenin. This finding provides evidence that miRNA-320 may be a potential biomarker of radiosensitivity in cervical cancer.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yang CX, Zhang SM, Li J, Yang B, Ouyang W, Mei ZJ, Chen J, Dai J, Ke S, Zhou FX, Zhou FX, et al: MicroRNA-320 regulates the radiosensitivity of cervical cancer cells C33AR by targeting β-catenin. Oncol Lett 12: 4983-4990, 2016.
APA
Yang, C., Zhang, S., Li, J., Yang, B., Ouyang, W., Mei, Z. ... Xie, C. (2016). MicroRNA-320 regulates the radiosensitivity of cervical cancer cells C33AR by targeting β-catenin. Oncology Letters, 12, 4983-4990. https://doi.org/10.3892/ol.2016.5340
MLA
Yang, C., Zhang, S., Li, J., Yang, B., Ouyang, W., Mei, Z., Chen, J., Dai, J., Ke, S., Zhou, F., Zhou, Y., Xie, C."MicroRNA-320 regulates the radiosensitivity of cervical cancer cells C33AR by targeting β-catenin". Oncology Letters 12.6 (2016): 4983-4990.
Chicago
Yang, C., Zhang, S., Li, J., Yang, B., Ouyang, W., Mei, Z., Chen, J., Dai, J., Ke, S., Zhou, F., Zhou, Y., Xie, C."MicroRNA-320 regulates the radiosensitivity of cervical cancer cells C33AR by targeting β-catenin". Oncology Letters 12, no. 6 (2016): 4983-4990. https://doi.org/10.3892/ol.2016.5340