Heat shock factor 2 is associated with the occurrence of lung cancer by enhancing the expression of heat shock proteins
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- Published online on: November 8, 2016 https://doi.org/10.3892/ol.2016.5368
- Pages: 5106-5112
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Copyright: © Zhong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Cancer is the leading cause of morbidity and mortality worldwide, among which lung cancer accounts for more mortalities than other cancers (1). The occurrence of lung cancer is associated with complicated factors, including family history, infection (2), air pollution and tobacco use (3). Numerous genes are involved in the occurrence of lung cancer. Among them, numerous members of heat shock proteins (HSPs) are overexpressed in a wide range of human cancers and are implicated in tumor cell proliferation, differentiation and invasion, and also recognition by the immune system (4).
The heat shock responses were identified by Ritossa in 1962 in Drosophila in response to elevated temperatures (5). The heat shock responses are ubiquitous, existing in all organisms to protect cells against harmful conditions, including heat shock, oxidative stress or inflammation (6,7). The synthesis of HSPs is the typical cellular response to stress. HSPs aid cells to facilitate refolding or degradation of misfolded and aggregated proteins induced by stress. HSPs are involved in numerous basic cell processes, including cell proliferation, cell differentiation and apoptosis. Cancer is characterized by an aberrant level of cell growth, cell differentiation and apoptosis. It was previously found that altered expression of HSPs has been reported in almost all classes of tumors. Increased levels of HSP27, relative to its level in non-transformed cells, have been detected in a number of cancers, such as breast cancer, endometrial cancer and leukemia (6,8). Elevated expression of members of the HSP70 family has also been reported in high-grade malignant tumors (9). HSP90 family members, including Hsp90α and Hsp90β, are overexpressed in numerous types of cancers (10,11).
A stress-responsive promoter element can be found upstream of the site of transcription initiation of HSP genes, and it is termed the heat shock element (HSE). Heat shock factors (HSFs) can bind to HSEs, and thus regulate the expression of HSPs (12). In total 3 HSFs (HSF1, HSF2 and HSF4) have been characterized in human cells (13). Among them, HSF1 has been associated with the occurrence of cancer (14). HSF4 is also associated with cancer, and inactivation of HSF4 induces cellular senescence and suppresses tumorigenesis in vivo (15). Thus, in the present study, the expression level of HSF2 in lung cancer was investigated and the cellular role of HSF2, including cell proliferation and cell migration, was characterized.
Materials and methods
Ethics
The present study was approved by the Medical Ethics Committee of Kunming University of Science and Technology (Kunming, China). Human samples were used in accordance with the requirements of Medical Ethics Committee of Kunming University of Science and Technology, under the guidelines of the World Medical Assembly (Declaration of Helsinki). Written informed consent was obtained from the patients' families.
Lung tissue samples
Lung specimens (n=50) were obtained from the tumor and an adjacent non-cancerous area ≥6 cm from the tumor tissues of 50 patients with lung cancer from Yunnan Province at the First People's Hospital of Yunnan Province (Kunming, China) between April 2014 and January 2015, as previously described (16,17). The non-neoplastic tissue was confirmed to lack tumor cell infiltration using histological analysis. The tissues were immediately placed in liquid nitrogen and stored at −80°C until use.
RNA extraction and polymerase chain reaction (PCR)
RNA extraction and first-strand cDNA synthesis was assessed as previously described (18). For quantitative PCR (qPCR) of HSF2, the following primers were used: HSF2 forward, 5′-AAGTTCAGGCAGTGATGGCA-3′ and reverse, 5′-TGCACAGAACTAGTGAAAAGATCA-3′; glyceraldehyde 3-phospahte dehydrogenase forward, 5′-GAAGGTCGGAGTCAACGGAT-3′ and reverse, 5′-GAGGGATCTCGCTCCTGGAAG-3′. qPCR was assessed using a continuous fluorescence detector (Opticon Monitor; Bio-Rad Laboratories Inc., Hercules, CA, USA) and PCR was assessed using an SYBR Green qPCR kit according to the manufacturer's protocol (Tiangen Bio, Inc., Beijing, China) with the following reaction conditions: Initial denaturation at 95°C for 1 min followed by 40 cycles at 95°C for 15 sec, 60°C for 15 sec and 72°C for 20 sec. Fluorescence curve analysis was assessed using Opticon Monitor software. The identities of qPCR products were confirmed by DNA sequencing. The relative gene expression in 2 different samples were compared using the 2−ΔΔCq method (19).
Western blot analysis
Western blot analysis of the tissues and the cells were performed as previously described (16,20,21). Tissue and cell samples were homogenized in radioimmunoprecipitation assay buffer containing a protease inhibitor cocktail (Roche Applied Science, Penzburg, Germany). Samples (containing 50 µg of protein) were loaded onto sodium dodecyl sulfate-polyacrylamide electrophoresis gel, electrophoresed and then electro-transferred onto a polyvinylidene fluoride membrane. The membrane was subsequently blocked with 3% bovine serum albumin and incubated with a rabbit anti-human HSF2 polyclonal antibody (dilution, 1:1,000; cat. no. SC-13056; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and a horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (dilution, 1:5,000; cat. no. SC-2004; Santa Cruz Biotechnology, Inc.). Protein bands were visualized using Super Signal reagents (Thermo Fisher Scientific, Inc., Waltham, MA, USA). For detection of HSP27, HSP47, HSP70 and HSP90, the same methods were used. The antibodies were purchased from Santa Cruz Biotechnology, Inc and listed as follows: Rabbit anti-human Hsp27 polyclonal antibody (dilution, 1:1,000; cat. no. SC-9012); rabbit anti-human Hsp47 polyclonal antibody (dilution, 1:1,000; cat. no. SC-8352); rabbit anti-human Hsp70 polyclonal antibody (dilution, 1:1,000; cat. no. SC-25837); and rabbit anti-human Hsp90 polyclonal antibody (dilution, 1:1,000; cat. no. SC-7947).
HSF2 overexpression plasmid construction
The cDNA of HSF2 was amplified from normal lung tissue using an RNA extraction kit (Tiangen Bio, Inc.) and the first strand cDNA sythesis kit (Takara Biotechnology Co., Ltd., Dalian, China). The PCR primers used were as follows: Forward, 5′-CGCGTTCGGGTGTAGAATTT-3′; and reverse, 5′-CATCCCACCCCCGATCTTTC-3′. The cDNA with XhoI and BamHI restriction sites and flag tag at N-terminal were amplified from the HSF2 cDNA with the following primers: Forward, 5′-AGATCTCGAGCCTGCGCCGCGTTAACAATGA-3′; and reverse, 5′-AGATGGATCCTTACTTATCGTCGTCATCCTTGTAATCTTAGCTATCAATAAGTGGCAT-3′ (bold and underlined nucleotides are the restriction site of the enzymes). The PCR products and the plasmid pIRES2-EGFP were digested with XhoI and BamHI (Takara Biotechnology Co., Ltd.) and ligated with T4 ligase (Takara Biotechnology Co., Ltd.) and transformed into Escherichia coli DH5a competent cells (Tiangen Bio, Inc.). The positive clones were confirmed by sequencing, then the plasmid was amplified and purified for cell transfection.
Cell culture and transfection
The BEAS-2B and A549 cells were cultured in DMEM/F12 with 10% fetal bovine serum (FBS). The cells were grown in a humidified atmosphere containing 5% CO2 at 37°C. Transfection of cells was performed using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) as previously described (22).
Cell proliferation assay
The cells were seeded onto a 96-well plate (1,000 cells/well) 24 h following transfection. Fresh medium supplemented with 10% FBS was added into the plates and changed every 24 h. The number of viable cells was estimated by the methyl thiazolyl tetrazolium (MTT) method every 24 h.
Cell migration assay
Cell migration was tested with a Boyden chamber assay, which utilized 24-well plates (Corning Incorporated, Corning, NY, USA) and Transwell plates (8 µm pore; EMD Millipore, Billerica, MA, USA). The bottom side of the Transwell plates were coated with collagen type 1 (10 µg/ml; Sigma-Aldrich; Merck Millipore, Darmstadt, Germany). Subsequent to being starved for 12 h with starvation medium (DMEM/F12) containing 0.25% bovine serum albumin, the cells were detached with cell dissociation buffer, then 1×105 cells were planted into the top of each chamber. Medium containing 1% FBS was added into the bottom of the chamber for 16 h. Migrated cells were dyed with crystal violet. Bound crystal violet was eluted with 1 ml 10% acetic acid and the migration activity was expressed as the value monitored at 586 nm of extraction.
Statistical analysis
Differences in the numerical data between the cancer and adjacent normal tissues were evaluated using the paired Wilcoxon test. The in vitro data were analyzed with Student's t-test for variance. Experimental values were expressed as the mean ± standard deviation. A value of P<0.05 was considered to indicate a statistically significant difference.
Results
HSF2 is upregulated in tumor tissues in lung cancer patients
A total of 50 matched normal lung and lung cancer tissues were analyzed with RT-qPCR to detect the expression level of HSF2 (Fig. 1A). The results showed that 76% (38/50) of the HSF2 expression levels in cancer tissues were upregulated compared with the normal lung tissues (P=0.0003; Fig. 1A). The protein levels of HSF2 were also detected by western blotting, and the results showed that the protein levels of HSF2 were upregulated, which is consistent with the results from RT-qPCR (Fig. 1B).
HSF2 overexpression enhances the cell proliferation and cell migration in human lung epithelial cells and lung cancer cells
Since HSF2 is upregulated in lung cancer, it is reasonable to infer that it may affect the basic routine of lung epithelial cells and cancer cells, including cell proliferation and cell migration. Thus, a plasmid encoding HSF2 was constructed and transfected into human lung epithelia cell line BEAS-2B (Fig. 2A). The growth of HSF2-overexpressing BEAS-2B cells was monitored by MTT assay. The results showed that the HSF2 overexpression markedly enhanced the proliferation ability of BEAS-2B cells (P=0.0006; Fig. 2C). The effect of HSF2 overexpression on the lung cancer A549 cell line was also investigated (Fig. 2B), and similar results were acquired (P=0.0021; Fig. 2D). Apart from cell proliferation, enhanced cell migration was another characteristic of tumorigenesis. Thus, cell migration was tested using a Boyden chamber assay. The results showed that HSF2 overexpression significantly promoted the cell migration of BEAS-2B cells and A549 cells (Fig. 2E and F).
HSF2 overexpression promotes the expression of HSPs
As the upstream regulator of HSP, HSF2 may regulate the expression level of HSP27, HSP47, HSP70 and HSP90. The present study additionally tested whether HSF2 overexpression can affect the expression of these HSPs. Subsequent to overexpression of HSF2 in BEAS-2B cells, the expression level of HSPs was semi-quantified by western blot analysis. All 4 HSPs, consisting of HSP27, HSP47, HSP70 and HSP90, were upregulated following HSF2 overexpression. Among them, HSP47 and HSP70 were only slightly upregulated, but the expression level of HSP27 and HSP90 were increased (Fig. 3A). However, HSF2 overexpression upregulated all 4 HSPs in A549 cells (Fig. 3B). These inconsistent results suggest that the regulation mechanism through which HSF2 affects the expression of HSPs may be different in normal epithelial cells and cancer cells.
HSF2 promoted cell proliferation and cell migration is dependent of its induced upregulation of HSP27 and HSP90
Since HSF2 overexpression can promote lung epithelial cell and lung cancer cell proliferation and migration, and at the same time upregulate HSP, particularly HSP27 and HSP90, it is reasonable to infer that HSP27 and HSP90 is pivotal for HSF2 functioning in promoting cell proliferation and cell migration. Thus, in the present study, HSP27 or HSP90 siRNA (or a combination of the two) were co-transfected with HSF2-overexpressing plasmids into BEAS-2B and A549 cells to assess the role of HSP27 and HSP90 in HSF2 function (Fig. 4A). The results showed that HSP27 and HSP90 RNA interference significantly attenuated HSF2 enhanced cell growth (Fig. 4B) and cell migration (Fig. 4D) on BEAS-2B cells. Similar results were obtained in A549 cells (Fig. 4C and E).
Discussion
Cancer is the leading cause of morbidity and mortality worldwide, and lung cancer accounts for a large proportion. Therefore, exploring the molecular and cellular mechanisms of lung cancer is important. Early screening strategies can be developed, and deciphering the mechanism underlying tumorigenesis may facilitate the development of new strategies to successfully treat cancer and cancer-associated diseases (23).
Increasing evidence suggests that changes in the cellular microenvironment contribute to tumorigenesis (24,25). There are numerous bodily responses to microenvironmental changes, including inflammation. Among these responses, heat shock responses are the most conserved among all types of life, from bacterial to mammalian (26). Heat shock responses are generally accepted to be associated with tumor growth and metastasis (4). Numerous studies have focused on the role of HSPs on tumor growth and metastasis (27–29). As the upstream regulators of HSPs, HSFs may be also involved in cancer. Among the 3 members of HSFs, HSF1 has been well characterized for its association with cancer. A clinical study showed that high levels of HSF1 are associated with poor prognosis in breast cancer (30). It is also reported that HSF1 is involved in tumor progression via regulation of hypoxia-inducible factor 1 and RNA-binding protein HuR (31). However, the other two members, HSF2 and HSF4, have not been well characterized in cancer. Thus, in the present study, the expression level of HSF2 in lung cancer tissues was detected in 50 lung cancer patients. It was found that HSF2 level is upregulated in the majority of lung cancer tissues, suggesting that HSF2 may be involved in lung cancer growth and/or metastasis.
HSF1 is activated in response to the traditional heat shock stimuli. However, it is generally accepted that HSF2 is not activated in response to classical stress stimuli, but under developmentally associated conditions (32,33). Coincidentally, with the recent progress in developmental biology and cancer biology, more and more similarities between early embryo development and tumorigenesis have been found (34). Thus, the upregulation of HSF2 in tumorigenesis complements current research. Therefore, in the next step, the biological effect of HSF2 on basic cellular effects was tested. The results showed that HSF2 promoted cellular proliferation and cellular migration in vitro, which may contribute to tumorigenesis in vivo.
The underlying mechanism of HSF2 promoting cell proliferation and cell migration may be complicated. A previous study showed that HSF2 binds to the HSP90, HSP27, HSP70 and c-Fos and modulates their expression (35). In the present study, it was assessed whether HSF2 affected the expression of these HSPs, and found that HSF2 overexpression indeed promoted the expression of HSP27, HSP47, HSP70 and HSP90, particularly HSP27 and HSP90. This may partially explain why HSF2 promotes cell proliferation and cell migration. The knockdown of HSP27 or HSP90 can significantly attenuate the HSF2 induced cell proliferation and cell migration. However, additional research is required to understand the role of HSF2 in cancer. The involvement of certain signaling pathways, including the MAP kinase pathway, which utilizes ERK1/2, P38 and JNK in HSF2-regulated cell proliferation and cell motility, may be investigated in additional studies. There are a limited number of studies about the HSF2 expression and human diseases. A previous study showed that HSF2 is upregulated in inflammatory bowel diseases by enhancing the production of inflammatory cytokines. Inflammation, particularly chronic inflammation, is closely associated with the occurrence of cancer. Whether it is the same or a similar condition in lung cancer requires investigation. Additional detailed studies are required to answer whether the upregulation of HSF is the cause or the result of lung cancer, which is important for understanding the association between HSF2 upregulation and lung cancer occurrence.
In conclusion, to the best of our knowledge, the present study showed for the first time that HSF2 is upregulated in lung cancer and may be involved in tumorigenesis. HSF2 asserts its biological function by upregulating the expression of HSP and consequently affecting cell proliferation and cell migration. Whether HSF2 acts alone or with other proteins may require additional investigation. In addition, whether HSF2 is a good diagnostic marker or drug target requires additional research.
Acknowledgements
This study was supported by Yunnan Department of Natural Science and Kunming Medical University (grant no., 2013FB197) and The First People's Hospital of Yunnan Province Internal Institutions (grant no., 2016NS204).
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