Open Access

CRKL overexpression promotes cell proliferation and inhibits apoptosis in endometrial carcinoma

  • Authors:
    • Le Cai
    • He Wang
    • Qing Yang
  • View Affiliations

  • Published online on: November 17, 2016     https://doi.org/10.3892/ol.2016.5394
  • Pages: 51-56
  • Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The v-Crk avian sarcoma virus CT10 oncogene homolog-like (CRKL) protein is important in cancer progression. However, its expression pattern and biological roles in human endometrial carcinoma remain unexplored. The potential mechanism of CRKL‑induced cancer progression is still unclear. The present study aimed to explore the expression pattern and biological roles of CRKL in human endometrial carcinoma. Using immunohistochemistry, it was observed that the CRKL protein was overexpressed in 50.5% (44/87) of endometrial carcinoma tissues. Plasmid transfection of CRKL into Ishikawa cells was performed, and CRKL overexpression promoted cell proliferation, colony formation and cell cycle transition in the transfected cells. In addition, CRKL overexpression inhibited cell apoptosis in Ishikawa cells treated with cisplatin, with decreased caspase‑3 and caspase‑9 cleavage. Further analysis revealed that CRKL upregulated the expression of cyclin D1, cyclin E, B cell lymphoma (Bcl)‑2 and survivin, and downregulated Bcl‑2 associated X protein expression. In conclusion, the present study demonstrated that CRKL overexpression in endometrial carcinoma contributes to malignant cell growth and resistance to apoptosis, possibly through Bcl-2.
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January-2017
Volume 13 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cai L, Wang H and Yang Q: CRKL overexpression promotes cell proliferation and inhibits apoptosis in endometrial carcinoma. Oncol Lett 13: 51-56, 2017.
APA
Cai, L., Wang, H., & Yang, Q. (2017). CRKL overexpression promotes cell proliferation and inhibits apoptosis in endometrial carcinoma. Oncology Letters, 13, 51-56. https://doi.org/10.3892/ol.2016.5394
MLA
Cai, L., Wang, H., Yang, Q."CRKL overexpression promotes cell proliferation and inhibits apoptosis in endometrial carcinoma". Oncology Letters 13.1 (2017): 51-56.
Chicago
Cai, L., Wang, H., Yang, Q."CRKL overexpression promotes cell proliferation and inhibits apoptosis in endometrial carcinoma". Oncology Letters 13, no. 1 (2017): 51-56. https://doi.org/10.3892/ol.2016.5394