Bovine lactoferricin P13 triggers ROS‑mediated caspase‑dependent apoptosis in SMMC7721 cells

Meng,Lixiang; Xu,Geliang; Li,Jiansheng; Liu,Wenbin; Jia,Weidong; Ma,Jinliang; Wei,Decheng

doi:10.3892/ol.2016.5415

Bovine lactoferricin P13 triggers ROS‑mediated caspase‑dependent apoptosis in SMMC7721 cells

Authors:
- Lixiang Meng
- Geliang Xu
- Jiansheng Li
- Wenbin Liu
- Weidong Jia
- Jinliang Ma
- Decheng Wei
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Affiliations: Department of Hepatic Surgery and Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, Affiliated Provincial Hospital of Anhui Medical University, Hefei, Anhui 230001, P.R. China, Department of General Surgery, Anhui Provincial Children's Hospital, Beijing Children's Hospital Group, Hefei, Anhui 230051, P.R. China
Published online on: November 22, 2016 https://doi.org/10.3892/ol.2016.5415
Pages: 511-517

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Abstract

Bovine lactoferricin P13 (LfcinB‑P13) is a peptide derived from LfcinB. In the present study, the effect of LfcinB‑P13 on the human liver cancer cell line SMMC7721 was investigated in vitro and in vivo. The results of the present study indicate that LfcinB‑P13 significantly decreased SMMC7721 cell viability in vitro (P=0.032 vs. untreated cells), while exhibiting low cytotoxicity in the wild‑type liver cell line L02. In addition, the rate of apoptosis in SMMC7721 cells was significantly increased following treatment with 40 and 60 µg/ml LfcinB‑P13 (P=0.0053 vs. the control group), which was associated with an increase in the level of reactive oxygen species (ROS) and the activation of caspase‑3 and ‑9. Furthermore, ROS chelation led to the suppression of LfcinB‑P13‑mediated caspase‑3 and ‑9 activation in SMMC7721 cells. LfcinB‑P13 was demonstrated to markedly inhibit tumor growth in an SMMC7721‑xenograft nude mouse model. The results of the present study indicate that LfcinB‑P13 is a novel candidate therapeutic agent for the treatment of liver cancer.

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