Open Access

Increased expression of S100A6 promotes cell proliferation in gastric cancer cells

  • Authors:
    • Xiao‑Hong Wang
    • Hong Du
    • Lin Li
    • Duan‑Fang Shao
    • Xi‑Yao Zhong
    • Ying Hu
    • Yi‑Qiang Liu
    • Xiao‑Fang Xing
    • Xiao‑Jing Cheng
    • Ting Guo
    • Shen Li
    • Zi‑Yu Li
    • Zhao‑De Bu
    • Xian‑Zi Wen
    • Lian‑Hai Zhang
    • Jia‑Fu Ji
  • View Affiliations

  • Published online on: November 22, 2016     https://doi.org/10.3892/ol.2016.5419
  • Pages: 222-230
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

S100A6 is involved in regulating the progression of cancer. S100A6 can regulate the dynamics of cytoskeletal constituents, cell growth and differentiation by interacting with binding or target proteins. The present study investigated whether S100A6 affects cell proliferation in gastric cancer cells by stimulating several downstream factors. Firstly, the expression and localization of S100A6 were investigated using immunohistochemical staining, an immunoelectron microscopy and laser confocal scanning. A ChIP‑Chip assay was performed to determine the downstream factors of S100A6 using promoter Chip analysis, including approximately the ‑800 to +200 regions around the transcription starting point. Polymerase chain reaction analysis was performed to confirm this. It was found that the intensity of S100A6 staining was markedly higher in the cytoplasm and nucleus, and its expression level correlated with that of the Ki67 protein. The overexpression of S100A6 also promoted cell proliferation in AGS and BGC823 cell lines, detected using a Cell Counting‑Kit 8 assay. In cells overexpressing S100A6, the expression levels of interleukin (IL)‑8, cyclin‑dependent kinase (CDK)5, CDK4, minichromosome maintenance complex component 7 (MCM7) and B‑cell lymphoma 2 (Bcl2) were noticeably increased. In conclusion, the increased expression of S100A6 promoted cell proliferation by regulating the expression levels of IL‑8, CDK5, CDK4, MCM7 and Bcl2 in gastric cancer cells.
View Figures
View References

Related Articles

Journal Cover

January-2017
Volume 13 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang XH, Du H, Li L, Shao DF, Zhong XY, Hu Y, Liu YQ, Xing XF, Cheng XJ, Guo T, Guo T, et al: Increased expression of S100A6 promotes cell proliferation in gastric cancer cells. Oncol Lett 13: 222-230, 2017.
APA
Wang, X., Du, H., Li, L., Shao, D., Zhong, X., Hu, Y. ... Ji, J. (2017). Increased expression of S100A6 promotes cell proliferation in gastric cancer cells. Oncology Letters, 13, 222-230. https://doi.org/10.3892/ol.2016.5419
MLA
Wang, X., Du, H., Li, L., Shao, D., Zhong, X., Hu, Y., Liu, Y., Xing, X., Cheng, X., Guo, T., Li, S., Li, Z., Bu, Z., Wen, X., Zhang, L., Ji, J."Increased expression of S100A6 promotes cell proliferation in gastric cancer cells". Oncology Letters 13.1 (2017): 222-230.
Chicago
Wang, X., Du, H., Li, L., Shao, D., Zhong, X., Hu, Y., Liu, Y., Xing, X., Cheng, X., Guo, T., Li, S., Li, Z., Bu, Z., Wen, X., Zhang, L., Ji, J."Increased expression of S100A6 promotes cell proliferation in gastric cancer cells". Oncology Letters 13, no. 1 (2017): 222-230. https://doi.org/10.3892/ol.2016.5419