Protective effects of sodium selenite supplementation against irradiation-induced damage in non-cancerous human esophageal cells

Puspitasari,Irma  M.; Yamazaki,Chiho; Abdulah,Rizky; Putri,Mirasari; Kameo,Satomi; Nakano,Takashi; Koyama,Hiroshi

doi:10.3892/ol.2016.5434

Protective effects of sodium selenite supplementation against irradiation-induced damage in non-cancerous human esophageal cells

Authors:
- Irma M. Puspitasari
- Chiho Yamazaki
- Rizky Abdulah
- Mirasari Putri
- Satomi Kameo
- Takashi Nakano
- Hiroshi Koyama
View Affiliations

Affiliations: Department of Public Health, Gunma University Graduate School of Medicine, Maebashi, Gunma 371‑8511, Japan, Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, West Java 45363, Indonesia, Department of Radiation Oncology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371‑8511, Japan
Published online on: November 24, 2016 https://doi.org/10.3892/ol.2016.5434
Pages: 449-454

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The administration of radioprotective compounds is one approach to preventing radiation damage in non-cancerous tissues. Therefore, radioprotective compounds are crucial in clinical radiotherapy. Selenium is a radioprotective compound that has been used in previous clinical studies of radiotherapy. However, evidence regarding the effectiveness of selenium in radiotherapy and the mechanisms underlying the selenium‑induced reduction of the side effects of radiotherapy remains insufficient. To further investigate the effectiveness of selenium in radiotherapy, the present study examined the protective effects of sodium selenite supplementation administered prior to X-ray radiation treatment in CHEK-1 non-cancerous human esophageal cells. Sodium selenite supplementation increased glutathione peroxidase 1 (GPx-1) activity in a dose‑ and time-dependent manner. The sodium selenite dose that induced the highest GPx-1 activity was determined to be 50 nM for 72 h prior to radiotherapy. The half-maximal inhibitory concentration of sodium selenite in CHEK-1 cells was 3.6 µM. Sodium selenite supplementation increased the survival rate of the cells in a dose‑dependent manner and enhanced the degree of cell viability at 72 h post‑irradiation (P<0.05). Combined treatment with 50 nM sodium selenite and 2 gray (Gy) X‑ray irradiation decreased the number of sub‑G1 cells from 5.9 to 4.2% (P<0.05) and increased the proportion of G1 cells from 58.8 to 62.1%, compared with 2 Gy X-ray irradiation alone; however, this difference was not statistically significant (P=1.00). Western blot analysis revealed that treatment with 2 Gy X-ray irradiation significantly increased the expression levels of cleaved poly (ADP‑ribose) polymerase (PARP; P<0.05). In addition, combined treatment with 50 nM sodium selenite and 2 Gy X‑ray irradiation reduced the expression levels of cleaved PARP protein, compared with 2 Gy X-ray irradiation alone; however, this reduction was not statistically significant (P=0.423). These results suggest that 50 nM sodium selenite supplementation administered for 72 h prior to irradiation may protect CHEK-1 cells from irradiation-induced damage by inhibiting irradiation‑induced apoptosis. Therefore, sodium selenite is a potential radioprotective compound for non‑cancerous cells in clinical radiotherapy.

View Figures

View References

1	Shirazi A, Ghobadi G and Ghazi-Khansari M: A radiobiological review on melatonin: A novel radioprotector. J Radiat Res. 48:263–272. 2007. View Article : Google Scholar : PubMed/NCBI
2	Greenberger JS: Radioprotection. Vivo. 23:323–336. 2009.
3	Nair CK, Parida DK and Nomura T: Radioprotectors in radiotherapy. J Radiat Res. 42:21–37. 2001. View Article : Google Scholar : PubMed/NCBI
4	Dörr W: Effects of selenium on radiation responses of tumor cells and tissue. Strahlenther Onkol. 182:693–695. 2006. View Article : Google Scholar : PubMed/NCBI
5	Weiss JF and Landauer MR: Radioprotection by antioxidants. Ann N Y Acad Sci. 899:44–60. 2000. View Article : Google Scholar : PubMed/NCBI
6	Rayman MP: The importance of selenium to human health. Lancet. 356:233–241. 2000. View Article : Google Scholar : PubMed/NCBI
7	Puspitasari IM, Abdulah R, Yamazaki C, Kameo S, Nakano T and Koyama H: Updates on clinical studies of selenium supplementation in radiotherapy. Radiat Oncol. 9:1252014. View Article : Google Scholar : PubMed/NCBI
8	Rayman MP: Selenium in cancer prevention: A review of the evidence and mechanism of action. Proc Nutr Soc. 64:527–542. 2005. View Article : Google Scholar : PubMed/NCBI
9	Fritz H, Kennedy D, Fergusson D, Fernandes R, Cooley K, Seely A, Sagar S, Wong R and Seely D: Selenium and lung cancer: A systematic review and meta analysis. PLoS One. 6:e262592011. View Article : Google Scholar : PubMed/NCBI
10	Abdulah R, Miyazaki K, Nakazawa M and Koyama H: Chemical forms of selenium for cancer prevention. J Trace Elem Med Biol. 19:141–150. 2005. View Article : Google Scholar : PubMed/NCBI
11	Ponholzer A, Struhal G and Madersbacher S: Frequent use of complementary medicine by prostate cancer patients. Eur Urol. 43:604–608. 2003. View Article : Google Scholar : PubMed/NCBI
12	Micke O, Buntzel J, Kisters K, Schafer U, Micke P and Mucke R: Complementary and alternative medicine in lung cancer patients: A neglected phenomenon? Front Radiat Ther Oncol. 42:198–205. 2010. View Article : Google Scholar : PubMed/NCBI
13	Schleicher UM, Cotarelo C Lopez, Andreopoulos D, Handt S and Ammon J: Radioprotection of human endothelial cells by sodium selenite. Med Klin (Munich). 94:(Suppl 3). 35–38. 1999.(In German). View Article : Google Scholar : PubMed/NCBI
14	Rodemann HP, Hehr T and Bamberg M: Relevance of the radioprotective effect of sodium selenite. Med Klin (Munich). 94:(Suppl 3). 39–41. 1999.(In German). View Article : Google Scholar : PubMed/NCBI
15	Micke O, Schomburg L, Buentzel J, Kisters K and Muecke R: Selenium in oncology: From chemistry to clinics. Molecules. 14:3975–3988. 2009. View Article : Google Scholar : PubMed/NCBI
16	Diamond AM, Dale P, Murray JL and Grdina DJ: The inhibition of radiation-induced mutagenesis by the combined effects of selenium and the aminothiol WR-1065. Mutat Res. 356:147–154. 1996. View Article : Google Scholar : PubMed/NCBI
17	Eckers JC, Kalen AL, Xiao W, Sarsour EH and Goswami PC: Selenoprotein P inhibits radiation-induced late reactive oxygen species accumulation and normal cell injury. Int J Radiat Oncol Biol Phys. 87:619–625. 2013. View Article : Google Scholar : PubMed/NCBI
18	Tak JK and Park JW: The use of ebselen for radioprotection in cultured cells and mice. Free Radic Biol Med. 46:1177–1185. 2009. View Article : Google Scholar : PubMed/NCBI
19	Abdulah R, Faried A, Kobayashi K, Yamazaki C, Suradji EW, Ito K, Suzuki K, Murakami M, Kuwano H and Koyama H: Selenium enrichment of broccoli sprout extract increases chemosensitivity and apoptosis of LNCaP prostate cancer cells. BMC Cancer. 9:4142009. View Article : Google Scholar : PubMed/NCBI
20	Lowry OH, Rosebrough NJ, Farr AL and Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem. 193:265–275. 1951.PubMed/NCBI
21	Paglia DE and Valentine WN: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 70:158–169. 1967.PubMed/NCBI
22	Buch K, Peters T, Nawroth T, Sänger M, Schmidberger H and Langguth P: Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay-a comparative study. Radiat Oncol. 7:12012. View Article : Google Scholar : PubMed/NCBI
23	Franken NA, Rodermond HM, Stap J, Haveman J and van Bree C: Clonogenic assay of cells in vitro. Nat Protoc. 1:2315–2319. 2006. View Article : Google Scholar : PubMed/NCBI
24	Kanda Y: Investigation of the freely available easy-to-use software ‘EZR’ for medical statistics. Bone Marrow Transplant. 48:452–458. 2013. View Article : Google Scholar : PubMed/NCBI
25	Rafferty TS, Green MH, Lowe JE, Arlett C, Hunter JA, Beckett GJ and McKenzie RC: Effects of selenium compounds on induction of DNA damage by broadband ultraviolet radiation in human keratinocytes. Br J Dermatol. 148:1001–1019. 2003. View Article : Google Scholar : PubMed/NCBI
26	Lee JH, Kim SY, Kil IS and Park JW: Regulation of ionizing radiation-induced apoptosis by mitochondrial NADP+-dependent isocitrate dehydrogenase. J Biol Chem. 282:13385–13341. 2007. View Article : Google Scholar : PubMed/NCBI
27	Yang Y, Zhao S and Song J: Caspase-dependent apoptosis and -independent poly(ADP-ribose) polymerase cleavage induced by transforming growth factor beta1. Int J Biochem Cell Biol. 36:223–234. 2004. View Article : Google Scholar : PubMed/NCBI
28	Baker RD, Baker SS, LaRosa K, Whitney C and Newburger PE: Selenium regulation of glutathione peroxidase in human hepatoma cell line Hep3B. Arch Biochem Biophys. 304:53–57. 1993. View Article : Google Scholar : PubMed/NCBI
29	Husbeck B, Peehl DM and Knox SJ: Redox modulation of human prostate carcinoma cells by selenite increases radiation-induced cell killing. Free Radic Biol Med. 38:50–57. 2005. View Article : Google Scholar : PubMed/NCBI