Human serum albumin-mediated apoptin delivery suppresses breast cancer cell growth in vitro and in vivo

Wu,Fang; Liu,Yizhi; Li,Jian; Hou,Lei; Lei,Fuxi; Huang,Shangke; Feng,Lu; Zhao,Xinhan

doi:10.3892/ol.2016.5470

Human serum albumin-mediated apoptin delivery suppresses breast cancer cell growth in vitro and in vivo

Authors:
- Fang Wu
- Yizhi Liu
- Jian Li
- Lei Hou
- Fuxi Lei
- Shangke Huang
- Lu Feng
- Xinhan Zhao
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Affiliations: Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Medical Oncology, Shaanxi Provincial Tumor Hospital, Xi'an, Shaanxi 710061, P.R. China, Department of Medical Imaging, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China, Department of Medical Oncology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China
Published online on: December 7, 2016 https://doi.org/10.3892/ol.2016.5470
Pages: 579-586
Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gene therapy is one of the most promising potential therapeutic strategies for many types of cancer. Cell apoptosis is an active, programmed physiological process of the body, and its disruption has been closely associated with the occurrence of tumor development. Apoptin is known to induce tumor cell apoptosis. In the present study, the MCF-7 breast cancer cell line was transfected with a human serum albumin (HSA) and apoptin expressing plasmid [HSA‑polyethylenimine (PEI)-pcDNA-Apoptin]. Reverse transcription‑quantitative polymerase chain reaction and western blotting were performed to detect the expression of apoptin in the transfected MCF‑7 cells, while MTT assays and flow cytometry were conducted to detect cell viability and apoptosis. Furthermore, hematoxylin and eosin staining was used to observe the morphology of xenografts from mice injected with MCF‑7 cells. It was demonstrated that the HSA‑PEI‑pcDNA‑Apoptin expression plasmid resulted in the upregulation of apoptin in MCF‑7 cells, and efficiently suppressed breast tumor growth in vivo. These findings indicated that the use of HSA as an apoptin expression vector has potential therapeutic benefits for cancer and confirms the requirement for the further evaluation of apoptin in clinical trials.

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