Effects of RNA silencing of matrix metalloproteinase-2 on the growth of esophageal carcinoma cells in vivo

Shen,Yu‑Guang; Feng,Wen; Xu,Yi‑Jun; Jiao,Na‑Na; Sun,Da‑Qiang; Qu,Wen‑Dong; Tang,Quan; Xiong,Wei; Tang,Yang; Xia,Yu; Cai,Qing‑Yong; Liu,Da‑Xing; Zhang,Xun; Xu,Gang; Liang,Gui‑You

doi:10.3892/ol.2016.5542

Effects of RNA silencing of matrix metalloproteinase-2 on the growth of esophageal carcinoma cells in vivo

Authors:
- Yu‑Guang Shen
- Wen Feng
- Yi‑Jun Xu
- Na‑Na Jiao
- Da‑Qiang Sun
- Wen‑Dong Qu
- Quan Tang
- Wei Xiong
- Yang Tang
- Yu Xia
- Qing‑Yong Cai
- Da‑Xing Liu
- Xun Zhang
- Gang Xu
- Gui‑You Liang
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Affiliations: Department of Thoracic and Cardiovascular Surgery, The First People’s Hospital of Zunyi, Zunyi, Guizhou 563003, P.R. China, Department of Pathology, Henan Tumor Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China, Thoracic Department, Tianjin Chest Hospital, Tianjin 300051, P.R. China, Department of Thoracic and Cardiovascular Surgery, Affiliated Hospital of Zunyi Medical College, Guizhou 563003, P.R. China
Published online on: December 27, 2016 https://doi.org/10.3892/ol.2016.5542
Pages: 1119-1124
Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Esophageal carcinoma is one of the most common malignancies in China. Previous studies reported that matrix metalloproteinases (MMPs) have important roles in the progression and invasion of numerous types of solid tumors. Among the MMPs, MMP‑2 has been closely associated with tumor growth and invasion. In the present study, a short hairpin RNA (shRNA) lentiviral expression vector targeting the MMP‑2 gene was constructed in order to observe the inhibitory effect of MMP‑2 gene silencing on the growth of the KYSE150 esophageal carcinoma cell line in vivo. Three small hairpin RNA sequences targeting MMP‑2 were designed and cloned into lentiviral vectors. Following transfection of the lentiviral vectors into KTSE150 cells, MMP‑2 mRNA and protein expression levels were examined by reverse transcription‑quantitative polymerase chain reaction and western blotting, and the growth rate of cells was analyzed by MTT assays. Subsequently, tumor growth was assessed in nude mice. Lentivirus‑mediated RNA interference effectively inhibited the expression of MMP‑2 mRNA and protein in KYSE150 esophageal carcinoma cells, and suppressed the growth of esophageal carcinoma cells in vivo. The results of the present study suggested that lentivirus‑mediated gene therapy targeting MMP‑2 may be an attractive strategy for the treatment of esophageal carcinoma and justifies the performance of further studies on the application of lentivirus vectors to cancer gene therapy.

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