K9(C4H4FN2O2)2Nd(PW11O39)2·25H2O induces apoptosis in human lung cancer A549 cells

  • Authors:
    • Rong‑Yao Xia
    • Ran‑Ran Zhang
    • Zhe Jiang
    • Ya‑Jiao Sun
    • Jing Liu
    • Fu‑Hui Chen
  • View Affiliations

  • Published online on: December 28, 2016     https://doi.org/10.3892/ol.2016.5543
  • Pages: 1348-1352
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Abstract

Lung cancer is the leading cause of cancer-associated mortality worldwide. The present study investigated the effects of K9(C4H4FN2O2)2Nd(PW11O39)2·25H2O (FNdPW), a chemically synthesized polyoxometalate that contains rare earth elements, on lung cancer growth, and explored the mechanism underlying its actions. The effects of FNdPW on the cell viability and apoptosis of human lung cancer A549 cells were measured using MTT assay, acridine orange/ethidium bromide staining and electron microscopy. The expression of apoptosis‑related proteins, including B‑cell lymphoma (Bcl)-2-associated death promoter (Bad), phosphorylated (p)‑Bad, X‑linked inhibitor of apoptosis (XIAP), apoptosis-inducing factor (AIF), Bcl‑2‑associated X protein (Bax) and Bcl‑2, was determined by western blotting. Caspase‑3 activity was measured using a caspase‑3 activity kit. After 72 h of incubation, FNdPW reduced cell viability and induced apoptosis in A549 cells in a concentration‑ and time‑dependent manner. FNdPW upregulated the pro‑apoptotic Bad and Bax proteins, and downregulated the anti‑apoptotic p‑Bad, Bcl‑2 and XIAP proteins. Furthermore, FNdPW also enhanced caspase‑3 activity and increased the protein level of AIF in A549 cells, which was independent of the caspase‑3 pathway. These events were associated with the regulation exerted by FNdPW on multiple targets involved in A549 cell proliferation. Therefore, FNdPW may be a novel drug for the treatment of lung cancer.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Xia RY, Zhang RR, Jiang Z, Sun YJ, Liu J and Chen FH: K9(C4H4FN2O2)2Nd(PW11O39)2·25H2O induces apoptosis in human lung cancer A549 cells. Oncol Lett 13: 1348-1352, 2017
APA
Xia, R., Zhang, R., Jiang, Z., Sun, Y., Liu, J., & Chen, F. (2017). K9(C4H4FN2O2)2Nd(PW11O39)2·25H2O induces apoptosis in human lung cancer A549 cells. Oncology Letters, 13, 1348-1352. https://doi.org/10.3892/ol.2016.5543
MLA
Xia, R., Zhang, R., Jiang, Z., Sun, Y., Liu, J., Chen, F."K9(C4H4FN2O2)2Nd(PW11O39)2·25H2O induces apoptosis in human lung cancer A549 cells". Oncology Letters 13.3 (2017): 1348-1352.
Chicago
Xia, R., Zhang, R., Jiang, Z., Sun, Y., Liu, J., Chen, F."K9(C4H4FN2O2)2Nd(PW11O39)2·25H2O induces apoptosis in human lung cancer A549 cells". Oncology Letters 13, no. 3 (2017): 1348-1352. https://doi.org/10.3892/ol.2016.5543