Aberrant KLK4 gene promoter hypomethylation in pediatric hepatoblastomas

  • Authors:
    • Baihui Liu
    • Ximao Cui
    • Shan Zheng
    • Kuiran Dong
    • Rui Dong
  • View Affiliations

  • Published online on: January 2, 2017     https://doi.org/10.3892/ol.2017.5558
  • Pages: 1360-1364
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Abstract

DNA methylation has a crucial role in cancer biology and has been recognized as an activator of oncogenes and inactivator of tumor suppressor genes, both of which are mechanisms for tumorigenesis. Kallikrein‑related peptidase 4 (KLK4), has been suggested to be an oncogene in various types of cancer. The aim of the present study was to assess the DNA methylation patterns of the KLK4 gene in cancerous samples harvested from patients with hepatoblastoma (HB). KLK4 mRNA expression levels were detected using reverse transcription‑quantitative polymerase chain reaction and assessed its DNA methylation patterns using high‑throughput mass spectrometry on a matrix‑assisted laser desorption/ionization time‑of‑flight mass array. A total of 10 HB and 10 normal liver tissue samples were obtained from patients with HB. The results of the present study showed that a significantly higher level of KLK4 mRNA expression levels were detected in HB tissues, as compared with the matched controls. Furthermore, the KLK4 gene promoter region was distinctively less methylated in the HB samples compared with the controls and negatively correlated with KLK4 mRNA expression levels. These findings indicate that aberrant methylation of KLK4 may contribute to its upregulated mRNA expression in HB.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu B, Cui X, Zheng S, Dong K and Dong R: Aberrant KLK4 gene promoter hypomethylation in pediatric hepatoblastomas. Oncol Lett 13: 1360-1364, 2017
APA
Liu, B., Cui, X., Zheng, S., Dong, K., & Dong, R. (2017). Aberrant KLK4 gene promoter hypomethylation in pediatric hepatoblastomas. Oncology Letters, 13, 1360-1364. https://doi.org/10.3892/ol.2017.5558
MLA
Liu, B., Cui, X., Zheng, S., Dong, K., Dong, R."Aberrant KLK4 gene promoter hypomethylation in pediatric hepatoblastomas". Oncology Letters 13.3 (2017): 1360-1364.
Chicago
Liu, B., Cui, X., Zheng, S., Dong, K., Dong, R."Aberrant KLK4 gene promoter hypomethylation in pediatric hepatoblastomas". Oncology Letters 13, no. 3 (2017): 1360-1364. https://doi.org/10.3892/ol.2017.5558