Downregulation of RASAL2 promotes the proliferation, epithelial‑mesenchymal transition and metastasis of colorectal cancer cells

  • Authors:
    • Zeming Jia
    • Weidong Liu
    • Liansheng Gong
    • Zhongfu Xiao
  • View Affiliations

  • Published online on: January 10, 2017     https://doi.org/10.3892/ol.2017.5581
  • Pages: 1379-1385
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Abstract

RAS protein activator like 2 (RASAL2) is a RAS‑GTPase‑activating protein and has recently been identified to be a tumor suppressor in various types of human cancer; however, the function of RASAL2 in colorectal carcinoma (CRC) remains unclear. In the present study, the function of RASAL2 in CRC cells was investigated using a RASAL2 loss‑of‑function cell model. RASAL2 short hairpin RNA was transfected into the human CRC cell lines LoVo, SW620 and HCT116, and the wild-type colon cell line NCM460. The subsequent downregulation of RASAL2 was evaluated using western blot and reverse transcription‑quantitative polymerase chain reaction analyses. It was observed that RASAL2 expression was significantly decreased in human CRC tissues and cell lines (P<0.01). In the loss‑of‑function cell models, RASAL2 expression was decreased significantly, while cell proliferation, colony formation, migration and invasion were increased (all P<0.01). These effects were associated with the induction of epithelial‑mesenchymal transition and Raf/mitogen‑activated protein kinase hyperactivation. The results of the present study indicate that RASAL2 is a potential therapeutic target to inhibit CRC progression and metastasis.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Jia Z, Liu W, Gong L and Xiao Z: Downregulation of RASAL2 promotes the proliferation, epithelial‑mesenchymal transition and metastasis of colorectal cancer cells. Oncol Lett 13: 1379-1385, 2017
APA
Jia, Z., Liu, W., Gong, L., & Xiao, Z. (2017). Downregulation of RASAL2 promotes the proliferation, epithelial‑mesenchymal transition and metastasis of colorectal cancer cells. Oncology Letters, 13, 1379-1385. https://doi.org/10.3892/ol.2017.5581
MLA
Jia, Z., Liu, W., Gong, L., Xiao, Z."Downregulation of RASAL2 promotes the proliferation, epithelial‑mesenchymal transition and metastasis of colorectal cancer cells". Oncology Letters 13.3 (2017): 1379-1385.
Chicago
Jia, Z., Liu, W., Gong, L., Xiao, Z."Downregulation of RASAL2 promotes the proliferation, epithelial‑mesenchymal transition and metastasis of colorectal cancer cells". Oncology Letters 13, no. 3 (2017): 1379-1385. https://doi.org/10.3892/ol.2017.5581