Effect of cigarette smoke exposure and mutant Kras overexpression on pancreatic cell proliferation

  • Authors:
    • Howard P. Glauert
    • R. Scott Elliott
    • Sung Gu Han
    • Mark Athey
    • Eun Y. Lee
    • C. Gary Gairola
  • View Affiliations

  • Published online on: January 17, 2017     https://doi.org/10.3892/ol.2017.5606
  • Pages: 1939-1943
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Abstract

Pancreatic cancer is the fourth leading cause of cancer‑associated mortality. The major risk factor for pancreatic cancer is cigarette smoking. Kras mutations are commonly observed in human pancreatic cancers. The present study examined the hypothesis that exposure to cigarette smoke and overexpression of a mutant Kras gene in the pancreas affects pancreatic cell proliferation in mice. Mice overexpressing the mutant Kras gene (KRasG12D) in the pancreas as well as wild‑type mice were exposed to environmental tobacco smoke for 2 weeks. Overexpression of mutant Kras increased cell proliferation in pancreatic ductal, acinar and islet cells. Notably, cigarette smoke exposure decreased cell proliferation in pancreatic ductal and acinar cells, and had no effect in islet cells. Cigarette smoke did not affect pancreatic protein levels of tumor necrosis factor (TNF)α, p53, or cyclin D1, but mutant Kras overexpression slightly decreased TNFα and p53 protein levels. Therefore, pancreatic cell proliferation in mice overexpressing mutant Kras is associated with the later development of pancreatic tumors, but effects of cigarette smoke on pancreatic cell proliferation do not provide a good model for human pancreatic carcinogenesis.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Glauert HP, Elliott RS, Han SG, Athey M, Lee EY and Gairola CG: Effect of cigarette smoke exposure and mutant Kras overexpression on pancreatic cell proliferation. Oncol Lett 13: 1939-1943, 2017.
APA
Glauert, H.P., Elliott, R.S., Han, S.G., Athey, M., Lee, E.Y., & Gairola, C.G. (2017). Effect of cigarette smoke exposure and mutant Kras overexpression on pancreatic cell proliferation. Oncology Letters, 13, 1939-1943. https://doi.org/10.3892/ol.2017.5606
MLA
Glauert, H. P., Elliott, R. S., Han, S. G., Athey, M., Lee, E. Y., Gairola, C. G."Effect of cigarette smoke exposure and mutant Kras overexpression on pancreatic cell proliferation". Oncology Letters 13.3 (2017): 1939-1943.
Chicago
Glauert, H. P., Elliott, R. S., Han, S. G., Athey, M., Lee, E. Y., Gairola, C. G."Effect of cigarette smoke exposure and mutant Kras overexpression on pancreatic cell proliferation". Oncology Letters 13, no. 3 (2017): 1939-1943. https://doi.org/10.3892/ol.2017.5606