Accumulation of low‑dose BIX01294 promotes metastatic potential of U251 glioblastoma cells

  • Authors:
    • Min Young Kim
    • Shin‑Ji Park
    • Jae Woong Shim
    • Yu Jin Song
    • Kwangmo Yang
    • Seong‑Joon Park
    • Kyu Heo
  • View Affiliations

  • Published online on: January 19, 2017     https://doi.org/10.3892/ol.2017.5626
  • Pages: 1767-1774
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Abstract

BIX01294 (Bix) is known to be a euchromatic histone‑lysine N‑methyltransferase 2 inhibitor and treatment with Bix suppresses cancer cell survival and proliferation. In the present study, it was observed that sequential treatment with low‑dose Bix notably increases glioblastoma cell migration and metastasis. It was demonstrated that U251 cells sequentially treated with low‑dose Bix exhibited induced characteristic changes in critical epithelial‑mesenchymal transition (EMT) markers, including E‑cadherin, N‑cadherin, β‑catenin and zinc finger protein SNAI2. Notably, sequential treatment with Bix also increased the expression of cancer stem cell‑associated markers, including sex determining region Y‑box 2, octamer‑binding transcription factor 4 and cluster of differentiation 133. Neurosphere formation was significantly enhanced in cells sequentially treated with Bix, compared with control cells (control: P=0.011; single treatment of Bix, P=0.045). The results of the present study suggest that accumulation of low‑dose Bix enhanced the migration and metastatic potential of glioblastoma cells by regulating EMT‑associated gene expression, which may be the cause of the altered properties of glioblastoma stem cells.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Kim MY, Park SJ, Shim JW, Song YJ, Yang K, Park SJ and Heo K: Accumulation of low‑dose BIX01294 promotes metastatic potential of U251 glioblastoma cells. Oncol Lett 13: 1767-1774, 2017.
APA
Kim, M.Y., Park, S., Shim, J.W., Song, Y.J., Yang, K., Park, S., & Heo, K. (2017). Accumulation of low‑dose BIX01294 promotes metastatic potential of U251 glioblastoma cells. Oncology Letters, 13, 1767-1774. https://doi.org/10.3892/ol.2017.5626
MLA
Kim, M. Y., Park, S., Shim, J. W., Song, Y. J., Yang, K., Park, S., Heo, K."Accumulation of low‑dose BIX01294 promotes metastatic potential of U251 glioblastoma cells". Oncology Letters 13.3 (2017): 1767-1774.
Chicago
Kim, M. Y., Park, S., Shim, J. W., Song, Y. J., Yang, K., Park, S., Heo, K."Accumulation of low‑dose BIX01294 promotes metastatic potential of U251 glioblastoma cells". Oncology Letters 13, no. 3 (2017): 1767-1774. https://doi.org/10.3892/ol.2017.5626