Effects of PTCs on nonsense‑mediated mRNA decay are dependent on PTC location

  • Authors:
    • Heegyum Moon
    • Xuexiu Zheng
    • Tiing Jen Loh
    • Ha Na Jang
    • Yongchao Liu
    • Da‑Woon Jung
    • Darren R. Williams
    • Haihong Shen
  • View Affiliations

  • Published online on: January 19, 2017     https://doi.org/10.3892/ol.2017.5627
  • Pages: 1944-1948
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Abstract

The récepteur d'origine nantais (RON) gene is a proto-oncogene that is responsible for encoding the human macrophage‑stimulating protein (MSP) 1 receptor. MSP activation induces RON‑mediated cell dissociation, migration and matrix invasion. Isoforms of RON that exclude exons 5 and 6 encode the RONΔ160 protein, which promotes cell transformation in vitro and tumor metastasis in vivo. Premature termination codons (PTCs) in exons activate the nonsense‑mediated mRNA decay (NMD) signaling pathway. The present study demonstrated that PTCs at various locations in the alternative exons 5 and 6 could induce NMD of the majority of the spliced, or partially spliced, isoforms. However, the isoforms that excluded exon 6 or exons 5 and 6 were markedly increased when produced from mutated minigenes with inserted PTCs. Furthermore, the unspliced isoform of intron 5 was not observed to be decreased by the presence of PTCs. Notably, these effects may be dependent on the location of the PTCs. The current study demonstrated a novel mechanism underlying the regulation of NMD in alternative splicing.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Moon H, Zheng X, Loh TJ, Jang HN, Liu Y, Jung DW, Williams DR and Shen H: Effects of PTCs on nonsense‑mediated mRNA decay are dependent on PTC location. Oncol Lett 13: 1944-1948, 2017
APA
Moon, H., Zheng, X., Loh, T.J., Jang, H.N., Liu, Y., Jung, D. ... Shen, H. (2017). Effects of PTCs on nonsense‑mediated mRNA decay are dependent on PTC location. Oncology Letters, 13, 1944-1948. https://doi.org/10.3892/ol.2017.5627
MLA
Moon, H., Zheng, X., Loh, T. J., Jang, H. N., Liu, Y., Jung, D., Williams, D. R., Shen, H."Effects of PTCs on nonsense‑mediated mRNA decay are dependent on PTC location". Oncology Letters 13.3 (2017): 1944-1948.
Chicago
Moon, H., Zheng, X., Loh, T. J., Jang, H. N., Liu, Y., Jung, D., Williams, D. R., Shen, H."Effects of PTCs on nonsense‑mediated mRNA decay are dependent on PTC location". Oncology Letters 13, no. 3 (2017): 1944-1948. https://doi.org/10.3892/ol.2017.5627