Open Access

miR-22 promotes apoptosis of osteosarcoma cells via inducing cell cycle arrest

  • Authors:
    • Pengzhou Gai
    • Hongliang Sun
    • Guangda Wang
    • Qiang Xu
    • Xiaojun Qi
    • Zuofu Zhang
    • Lei Jiang
  • View Affiliations

  • Published online on: February 2, 2017     https://doi.org/10.3892/ol.2017.5674
  • Pages: 2354-2358
  • Copyright: © Gai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To study the effects of miR-22 on the proliferation and the apoptosis of osteosarcoma MG-63 cell line and to explore the potential molecular mechanism that miR-22 regulates this biological process. Quantitive real-time polymerase chain reaction (RT-qPCR) was performed to explore the miRNA level of miR-22. The MG-63 cell line was infected with miR-22 mimics for establishment of miR-22 overexpression. Non-infected cells were in blank group and cells infected with empty vector were served as negative control (NC group). MTT assay was conducted to measure cell viability. The cell cycle and apoptosis were explored using flow cytometry and the apoptosis-related markers were detected by western blotting. RT-qPCR results revealed that the miR-22 miRNA level in the MG-63 cells was significantly lower than that in osteoblasts (P<0.05). MTT assay showed that the MG-63 cells infected with miR-22 mimics exhibited markedly decreased proliferation ability compared with blank and empty vector (NC) groups. Next, we found that overexpression of miR-22 remarkably increased the apoptosis of the MG-63 cells, evidenced from the flow cytometry results and elevated Bax and reduced Bcl-2. Furthermore, results revealed that percentage of the cells at G0/G1 phase in miR-22 mimic group (66.75±3.67%) was significantly higher than blank (52.9±2.58%) and NC (50.5±2.45%) groups. miR-22 attenuated the proliferation and induced the apoptosis of the MG-63 cells via promoting G0/G1 cell cycle arrest. Thus, miR-22 may have the potential to be a novel therapeutic in treatment of osteosarcoma.

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April-2017
Volume 13 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Gai P, Sun H, Wang G, Xu Q, Qi X, Zhang Z and Jiang L: miR-22 promotes apoptosis of osteosarcoma cells via inducing cell cycle arrest. Oncol Lett 13: 2354-2358, 2017
APA
Gai, P., Sun, H., Wang, G., Xu, Q., Qi, X., Zhang, Z., & Jiang, L. (2017). miR-22 promotes apoptosis of osteosarcoma cells via inducing cell cycle arrest. Oncology Letters, 13, 2354-2358. https://doi.org/10.3892/ol.2017.5674
MLA
Gai, P., Sun, H., Wang, G., Xu, Q., Qi, X., Zhang, Z., Jiang, L."miR-22 promotes apoptosis of osteosarcoma cells via inducing cell cycle arrest". Oncology Letters 13.4 (2017): 2354-2358.
Chicago
Gai, P., Sun, H., Wang, G., Xu, Q., Qi, X., Zhang, Z., Jiang, L."miR-22 promotes apoptosis of osteosarcoma cells via inducing cell cycle arrest". Oncology Letters 13, no. 4 (2017): 2354-2358. https://doi.org/10.3892/ol.2017.5674