miR-215 suppresses proliferation and migration of non-small cell lung cancer cells
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- Published online on: February 7, 2017 https://doi.org/10.3892/ol.2017.5692
- Pages: 2349-2353
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Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
The expression of microRNA-215 (miR-215) in non-small cell lung cancer (NSCLC) tissues and the effects of miR‑215 on the proliferation and migration of NSCLC cells were investigated. qRT‑PCR was used to detect the expression of miR‑215 in NSCLC tissues and paired normal tumor‑adjacent lung tissues; MTT assay, transwell assay and soft‑agar assay were used in vitro to evaluate the role of miR‑215 on proliferation, migration and cell clonality on NSCLC cells, after transfecting miR‑215 mimics to NSCLC cell line A549 or miR‑215 to H1299. miR‑215 was significantly decreased in NSCLC tissues compared to the paired normal tissues; Overexpression of miR‑215 in A549 cells resulted in reduction of the cell proliferation, migration and cell clonality, while downregulation of miR‑215 in H1299 cells could promote cell proliferation, migration and clonality. In conclusion, miR‑215 was downregulated in NSCLC tissues and may play a key role in the development of NSCLC.
