Polyphenolic compounds from Korean Lonicera japonica Thunb. induces apoptosis via AKT and caspase cascade activation in A549 cells
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- Published online on: February 23, 2017 https://doi.org/10.3892/ol.2017.5771
- Pages: 2521-2530
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Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
Lonicera japonica Thunb. (L. japonica T.) has historically been used in Korean herbal medicine due to its anticancer and protective effects on the respiratory system. In the present study, the polyphenolic compounds in L. japonica T. were investigated using high-performance liquid chromatography coupled with tandem mass spectrometry, and its anticancer effects on A549 non‑small‑cell lung cancer cells were studied. Polyphenolic compounds potentially inhibit A549 cells in a dose-dependent manner. Flow cytometry and western blot analysis demonstrated that polyphenolic compounds induce apoptosis by regulating the protein expression levels of caspases, poly‑(ADP‑ribose) polymerase and the B‑cell lymphoma‑2‑associated X-protein/B‑cell lymphoma‑extra large ratio. Furthermore, polyphenolic compounds inhibited mitochondrial membrane potential activity. Caspase‑3 activity was increased in a dose‑dependent manner and polyphenolic compounds inhibited the activation of protein kinase B by dephosphorylation. These results suggest that polyphenolic compounds in A549 cells indicate the anticancer activity through the induction of apoptosis.
