Expression of claudin 1, 4 and 7 in thyroid neoplasms

Süren,Di̇nç; Yildirim,Mustafa; Sayi̇ner,Alper; Ali̇kanoğlu,Arsenal Sezgi̇n; Atalay,İrem; Gündüz,Umut Riza; Kaya,Vi̇ldan; Gündüz,Şeyda; Oruç,Mehmet Tahi̇r; Sezer,Cem

doi:10.3892/ol.2017.5916

Expression of claudin 1, 4 and 7 in thyroid neoplasms

Authors:
- Di̇nç Süren
- Mustafa Yildirim
- Alper Sayi̇ner
- Arsenal Sezgi̇n Ali̇kanoğlu
- İrem Atalay
- Umut Riza Gündüz
- Vi̇ldan Kaya
- Şeyda Gündüz
- Mehmet Tahi̇r Oruç
- Cem Sezer
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Affiliations: Department of Pathology, University of Health Sciences, Antalya Education and Research Hospital, Antalya 07050, Turkey, Department of Medical Oncology, Medical Park Hospital, Gaziantep 27100, Turkey, Department of General Surgery, University of Health Sciences, Antalya Education and Research Hospital, Antalya 07050, Turkey, Department of Radiation Oncology, Süleyman Demirel University, Isparta 32260, Turkey, Department of Medical Oncology, University of Health Sciences, Antalya Education and Research Hospital, Antalya 07050, Turkey
Published online on: March 27, 2017 https://doi.org/10.3892/ol.2017.5916
Pages: 3722-3726

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Abstract

The distinction of thyroid carcinoma from benign thyroid neoplasm, as well as the subtyping of papillary carcinoma (PC) and follicular carcinoma (FC), may be performed histopathologically in the majority of cases. However, in certain cases, it is difficult to histopathologically distinguish between PC and FC, as well as follicular adenoma (FA), FC and the dominant nodule of multinodular goiter (MNG‑DN). The present study aimed to determine the roles of the expression levels of the tight junction proteins claudin 1, 4 and 7 in the differential diagnosis of PC, FC, FA, MNG‑DN, medullary carcinoma (MC) and anaplastic carcinoma (AC). The current study included 114 cases of histopathologically diagnosed thyroid neoplasia, which were distributed as follows: 29 FA, 18 MNG‑DN, 47 PC, 10 FC, 5 MC and 5 AC. The expression levels of claudin 1, 4 and 7 were examined using immunohistochemical methods. The results revealed a significant difference in claudin 1 expression between malignant and benign thyroid neoplasms (P<0.001). Claudin 1 expression was not detected in any of the MNG‑DN cases, and no significant difference in claudin 1 expression levels was identified between FA and FC (P=0.653). However, a statistically significant difference was observed between FC and PC (P<0.001). Claudin 4 expression did not differ between malignant and benign thyroid neoplasms, neither between MNG‑DN, FA and FC, nor between FC and PC (P=0.068, P=0.502 and P=0.481, respectively). Claudin 7 exhibited positive immunohistochemical staining in 107 patients (94%); however, no significant difference in claudin 7 expression §levels was identified between malignant and benign thyroid neoplasms among MNG‑DN, FA and FC (malignant, P=0.135; benign, P=0.470). Claudin 7 exhibited positive staining in all PC and FC cases. Therefore, claudin 1 expression levels may be useful in distinguishing cases of FC and PC with overlapping histopathological features, and provide a novel immunohistochemical marker for the subtyping of thyroid carcinoma.

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