Andrographolide induces apoptotic and non‑apoptotic death and enhances tumor necrosis factor‑related apoptosis‑inducing ligand‑mediated apoptosis in gastric cancer cells

  • Authors:
    • Sung‑Chul Lim
    • Ho Jong Jeon
    • Keun Hong Kee
    • Mi Ja Lee
    • Ran Hong
    • Song Iy Han
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  • Published online on: March 27, 2017     https://doi.org/10.3892/ol.2017.5923
  • Pages: 3837-3844
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Abstract

Andrographolide, a natural compound isolated from Andrographis paniculata, has been reported to possess antitumor activity. In the present study, the effect of andrographolide in human gastric cancer (GC) cells was investigated. Andrographolide induced cell death with apoptotic and non‑apoptotic features. At a low concentration, andrographolide potentiated apoptosis and reduction of clonogenicity triggered by recombinant human tumor necrosis factor‑related apoptosis‑inducing ligand (rhTRAIL). Exposure of GC cells to andrographolide altered the expression level of several growth‑inhibiting and apoptosis‑regulating proteins, including death receptors. It was demonstrated that activity of the TRAIL‑R2 (DR5) pathway was critical in the development of andrographolide‑mediated rhTRAIL sensitization, since its inhibition significantly reduced the extent of apoptosis induced by the combination of rhTRAIL and andrographolide. In addition, andrographolide increased reactive oxygen species (ROS) generation in a dose‑dependent manner. N‑acetyl cysteine prevented andrographolide‑mediated DR5 induction and the apoptotic effect induced by the combination of rhTRAIL and andrographolide. Collectively, the present study demonstrated that andrographolide enhances TRAIL‑induced apoptosis through induction of DR5 expression. This effect appears to involve ROS generation in GCs.
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May-2017
Volume 13 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lim SC, Jeon HJ, Kee KH, Lee MJ, Hong R and Han SI: Andrographolide induces apoptotic and non‑apoptotic death and enhances tumor necrosis factor‑related apoptosis‑inducing ligand‑mediated apoptosis in gastric cancer cells. Oncol Lett 13: 3837-3844, 2017.
APA
Lim, S., Jeon, H.J., Kee, K.H., Lee, M.J., Hong, R., & Han, S.I. (2017). Andrographolide induces apoptotic and non‑apoptotic death and enhances tumor necrosis factor‑related apoptosis‑inducing ligand‑mediated apoptosis in gastric cancer cells. Oncology Letters, 13, 3837-3844. https://doi.org/10.3892/ol.2017.5923
MLA
Lim, S., Jeon, H. J., Kee, K. H., Lee, M. J., Hong, R., Han, S. I."Andrographolide induces apoptotic and non‑apoptotic death and enhances tumor necrosis factor‑related apoptosis‑inducing ligand‑mediated apoptosis in gastric cancer cells". Oncology Letters 13.5 (2017): 3837-3844.
Chicago
Lim, S., Jeon, H. J., Kee, K. H., Lee, M. J., Hong, R., Han, S. I."Andrographolide induces apoptotic and non‑apoptotic death and enhances tumor necrosis factor‑related apoptosis‑inducing ligand‑mediated apoptosis in gastric cancer cells". Oncology Letters 13, no. 5 (2017): 3837-3844. https://doi.org/10.3892/ol.2017.5923