Overexpression of the anti-apoptotic protein BAG3 in human choroidal melanoma: A case report

Yunoki,Tatsuya; Tabuchi,Yoshiaki; Kondo,Takashi; Ishii,Yoko; Hayashi,Atsushi

doi:10.3892/ol.2017.5958

Overexpression of the anti-apoptotic protein BAG3 in human choroidal melanoma: A case report

Authors:
- Tatsuya Yunoki
- Yoshiaki Tabuchi
- Takashi Kondo
- Yoko Ishii
- Atsushi Hayashi
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Affiliations: Department of Ophthalmology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930‑0194, Japan, Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Toyama 930‑0194, Japan, Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930‑0194, Japan, Department of Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930‑0194, Japan
Published online on: March 30, 2017 https://doi.org/10.3892/ol.2017.5958
Pages: 4169-4172
Copyright: © Yunoki et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bcl-2-associated athanogene 3 (BAG3), a co-chaperone of heat shock protein 70 (HSP70), exerts anti-apoptotic effects in various malignant tumors. However, relationships between choroidal melanoma and BAG3 are poorly studied. This study investigated the expression of BAG3 in a case of human choroidal melanoma. Funduscopy, computed tomography, and single-photon emission computed tomography with the intravenous injection of N‑isopropyl‑p‑[123I] iodoamphetamine strongly indicated choroidal melanoma in a 68‑year‑old woman. Accordingly, we carried out an enucleation and pathological diagnosis. Proteins and total RNA were extracted from normal retinochoroidal and tumor tissues. Proteins were also extracted from ocular nevus tissues of other patients. We examined the expression of BAG3 protein and mRNA using Western blotting and the real‑time quantitative polymerase chain reaction, respectively. Immunohistochemical stains were positive for melan-A, HMB‑45, and S‑100. Histopathology confirmed a choroidal melanoma. The expression of BAG3 protein and mRNA in the choroidal melanoma tissue was upregulated with respect to both normal retinochoroidal tissue and ocular nevus tissues from other patients. Because BAG3 may inhibit apoptosis of choroidal melanoma and facilitate its survival, overexpression of this gene product may be a prognostic marker and therapeutic target.

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