Promoter methylation status of the tumor suppressor genes p16 and cadherin 1 in cervical intraepithelial neoplasia

  • Authors:
    • Ruei‑Nian Li
    • Chien‑Yu Li
    • Chien‑Hung Lee
    • Chiung‑Yu Peng
    • Ming‑Tsang Wu
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  • Published online on: April 3, 2017     https://doi.org/10.3892/ol.2017.5975
  • Pages: 4397-4401
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Abstract

Cervical cancer is the second most common female cancer worldwide. DNA methylation is one of a number of epigenetic regulation mechanisms leading to gene silencing in neoplastic cells. Aberrant methylation results in the silencing of tumor suppressor gene expression, and has been detected in a high percentage of human cancers. In the present study, the methylation status of three tumor suppressor genes, retinoic acid receptor β (RARβ), p16 and cadherin 1 (CDH1), and the inflammatory-associated cyclooxygenase-2 (COX‑2) gene, was examined at distinct stages of cervical intraepithelial neoplasia (CIN). The results of the present study revealed that the COX‑2 gene was unmethylated between CIN I and carcinoma specimens. The RARβ gene exhibited a minimal change in methylation frequency, whereas the CDH1 methylation level was increased <2‑fold between CIN I and carcinoma. Notably, the methylation frequency of p16 was 13.2% in normal specimens; 18.2% in CIN I; 35.7% in CIN II; 31.6% in CIN III; and 15.4% in carcinoma. By contrast, the methylation frequency of p16 increased between CIN I and carcinoma in the absence of high‑risk group papillomaviruses. The results of bisulfite sequencing indicated that the 10 CpG sites were all methylated in p16 gene methylation‑positive individuals. The results of the present study demonstrate that the methylation frequency of p16 and CDH1 was progressively increased during the development of malignant stages in CIN, and may be an additional tool for current cytomorphology-based screening of cervical cell specimens.
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June-2017
Volume 13 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li RN, Li CY, Lee CH, Peng CY and Wu MT: Promoter methylation status of the tumor suppressor genes p16 and cadherin 1 in cervical intraepithelial neoplasia. Oncol Lett 13: 4397-4401, 2017
APA
Li, R., Li, C., Lee, C., Peng, C., & Wu, M. (2017). Promoter methylation status of the tumor suppressor genes p16 and cadherin 1 in cervical intraepithelial neoplasia. Oncology Letters, 13, 4397-4401. https://doi.org/10.3892/ol.2017.5975
MLA
Li, R., Li, C., Lee, C., Peng, C., Wu, M."Promoter methylation status of the tumor suppressor genes p16 and cadherin 1 in cervical intraepithelial neoplasia". Oncology Letters 13.6 (2017): 4397-4401.
Chicago
Li, R., Li, C., Lee, C., Peng, C., Wu, M."Promoter methylation status of the tumor suppressor genes p16 and cadherin 1 in cervical intraepithelial neoplasia". Oncology Letters 13, no. 6 (2017): 4397-4401. https://doi.org/10.3892/ol.2017.5975