Open Access

DNA topoisomerase IIα and Ki67 are prognostic factors in patients with hepatocellular carcinoma

  • Authors:
    • Yi Cao
    • Ruisheng Ke
    • Shaohu Wang
    • Xu Zhu
    • Jianwei Chen
    • Chao Huang
    • Yi Jiang
    • Lizhi Lv
  • View Affiliations

  • Published online on: April 6, 2017     https://doi.org/10.3892/ol.2017.5999
  • Pages: 4109-4116
  • Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study was designed to determine the significance of DNA topoisomerase IIa (TopoIIα) and Ki67 in hepatocellular carcinoma cells (HCCs). The present study included 353 patients with HCC. The association of clinicopathological data with the expression of TopoIIα and Ki67 by immunohistochemistry was analyzed by χ2 test. Cox multivariate proportional hazards regression analysis and Kaplan‑Meier analysis were performed with all the variables to derive risk estimates associated with overall survival (OS)/recurrence‑free survival (RFS) and to control for confounders. TopoIIα and Ki67 were detected in the nuclei of the tumor cells. With TopoIIα, 35.7% of cells exhibited high expression, which was associated with tumor‑node‑metastasis stage, tumor size and α‑fetoprotein level. With Ki67, 37.1% of cells exhibited high expression, which was associated with tumor‑node‑metastasis stage, tumor size and α-fetoprotein level. Correlation was identified between the expression level of TopoIIα and Ki67 in HCCs (r=0.444). Multivariate analysis revealed that high TopoIIα expression is a prognostic indicator for RFS [hazard ratio (HR), 2.002; 95% confidence interval (CI), 1.429‑2.806] and OS (HR, 2.749; 95% CI, 1.919‑3.939), and high Ki67 expression is a prognostic indicator for OS (HR, 1.816; 95% CI, 1.273‑2.589). The TopoIIα‑low group had a significantly increased RFS rate (55.6 vs. 31.7%) and OS rate (66.5 vs. 23.8%) compared with the TopoIIα‑high group. The OS rate was increased in the Ki67‑low group compared with the Ki67‑high group (67.0 vs. 26.5%). Expression of TopoIIα and Ki67 are independent prognostic factors for survival in HCCs. TopoIIα was positively associated with Ki67 expression.
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June-2017
Volume 13 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cao Y, Ke R, Wang S, Zhu X, Chen J, Huang C, Jiang Y and Lv L: DNA topoisomerase IIα and Ki67 are prognostic factors in patients with hepatocellular carcinoma. Oncol Lett 13: 4109-4116, 2017.
APA
Cao, Y., Ke, R., Wang, S., Zhu, X., Chen, J., Huang, C. ... Lv, L. (2017). DNA topoisomerase IIα and Ki67 are prognostic factors in patients with hepatocellular carcinoma. Oncology Letters, 13, 4109-4116. https://doi.org/10.3892/ol.2017.5999
MLA
Cao, Y., Ke, R., Wang, S., Zhu, X., Chen, J., Huang, C., Jiang, Y., Lv, L."DNA topoisomerase IIα and Ki67 are prognostic factors in patients with hepatocellular carcinoma". Oncology Letters 13.6 (2017): 4109-4116.
Chicago
Cao, Y., Ke, R., Wang, S., Zhu, X., Chen, J., Huang, C., Jiang, Y., Lv, L."DNA topoisomerase IIα and Ki67 are prognostic factors in patients with hepatocellular carcinoma". Oncology Letters 13, no. 6 (2017): 4109-4116. https://doi.org/10.3892/ol.2017.5999