lncRNA C22orf32-1 contributes to the tumorigenesis of nasopharyngeal carcinoma

Nie,Guo‑Hui; Li,Zhao; Duan,Hong‑Fang; Luo,Liang; Hu,Hong‑Yi; Yang,Wei‑Qiang; Nie,Li‑Ping; Zhu,Ru‑Fei; Chen,Xiao‑Fan; Zhang,Wei

doi:10.3892/ol.2017.6021

lncRNA C22orf32-1 contributes to the tumorigenesis of nasopharyngeal carcinoma

Authors:
- Guo‑Hui Nie
- Zhao Li
- Hong‑Fang Duan
- Liang Luo
- Hong‑Yi Hu
- Wei‑Qiang Yang
- Li‑Ping Nie
- Ru‑Fei Zhu
- Xiao‑Fan Chen
- Wei Zhang
View Affiliations

Affiliations: Department of Otolaryngology, Shenzhen Second People's Hospital, Shenzhen, Guangdong 518035, P.R. China, Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China, Department of Otolaryngology, Shenzhen Children's Hospital, Shenzhen, Guangdong 518038, P.R. China, Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China, Biomedical Research Institute, Shenzhen Peking University‑The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China
Published online on: April 11, 2017 https://doi.org/10.3892/ol.2017.6021
Pages: 4487-4492

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The mechanism of nasopharyngeal carcinoma (NPC) remains unclear. The present study investigated the abnormal expression of long non-coding (lnc)RNAs in NPC tissues and one NPC cell line to identify the involvement of lncRNAs in the tumorigenesis of NPC. Using a quantitative reverse transcription polymerase chain reaction (RT‑qPCR), the expression of lncRNA C22orf32-1 in NPC tissues and an NPC cell line was verified. The effects of lncRNA C22orf32‑1 on NPC cells were investigated with a cell proliferation assay, cell scratch assay, Transwell assay and a cell apoptosis assay. The expression levels of lncRNA C22orf32‑1 in NPC tissues and an NPC cell line were upregulated. lncRNA C22orf32‑1 promoted the proliferation, migration and invasion of NPC cells, and reduced the apoptosis of NPC cells. The data demonstrated that lncRNA C22orf32‑1 may facilitate the tumorigenesis of NPC, and may be used for the early diagnosis and treatment of NPC.

View Figures

View References

1	Agulnik M and Epstein JB: Nasopharyngeal carcinoma: Current management, future directions and dental implications. Oral Oncol. 44:617–627. 2008. View Article : Google Scholar : PubMed/NCBI
2	Lee AW, Lin JC and Ng WT: Current management of nasopharyngeal cancer. Semin Radiat Oncol. 22:233–244. 2012. View Article : Google Scholar : PubMed/NCBI
3	Spano JP, Busson P, Atlan D, Bourhis J, Pignon JP, Esteban C and Armand JP: Nasopharyngeal carcinomas: An update. Eur J Cancer. 39:2121–2135. 2003. View Article : Google Scholar : PubMed/NCBI
4	Vokes EE, Liebowitz DN and Weichselbaum RR: Nasopharyngeal carcinoma. Lancet. 350:1087–1091. 1997. View Article : Google Scholar : PubMed/NCBI
5	August M, Dodson TB, Nastri A and Chuang SK: Nasopharyngeal carcinoma: Clinical assessment and review of 176 cases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 91:205–214. 2001. View Article : Google Scholar : PubMed/NCBI
6	Chen W and Hu GH: Biomarkers for enhancing the radiosensitivity of nasopharyngeal carcinoma. Cancer Biol Med. 12:23–32. 2015.PubMed/NCBI
7	Wolff HA, Rödel RM, Gunawan B, Overbeck T, Herrmann MK, Hennies S, Hille A, Vorwerk H, Matthias C, Hess CF and Christiansen H: Nasopharyngeal carcinoma in adults: Treatment results after long-term follow-up with special reference to adjuvant interferon-beta in undifferentiated carcinomas. J Cancer Res Clin Oncol. 136:89–97. 2010. View Article : Google Scholar : PubMed/NCBI
8	Chou J, Lin YC, Kim J, You L, Xu Z, He B and Jablons DM: Nasopharyngeal carcinoma-review of the molecular mechanisms of tumorigenesis. Head Neck. 30:946–963. 2008. View Article : Google Scholar : PubMed/NCBI
9	Chen ZT, Liang ZG and Zhu XD: A Review: Proteomics in nasopharyngeal carcinoma. Int J Mol Sci. 16:15497–15530. 2015. View Article : Google Scholar : PubMed/NCBI
10	Razak AR, Siu LL, Liu FF, Ito E, O'Sullivan B and Chan K: Nasopharyngeal carcinoma: The next challenges. Eur J Cancer. 46:1967–1978. 2010. View Article : Google Scholar : PubMed/NCBI
11	Tulalamba W and Janvilisri T: Nasopharyngeal carcinoma signaling pathway: An update on molecular biomarkers. Int J Cell Biol. 2012:5946812012. View Article : Google Scholar : PubMed/NCBI
12	Morris KV and Mattick JS: The rise of regulatory RNA. Nat Rev Genet. 15:423–437. 2014. View Article : Google Scholar : PubMed/NCBI
13	Yarmishyn AA and Kurochkin IV: Long noncoding RNAs: A potential novel class of cancer biomarkers. Front Genet. 6:1452015. View Article : Google Scholar : PubMed/NCBI
14	Ulitsky I and Bartel DP: lincRNAs: Genomics, evolution, and mechanisms. Cell. 154:26–46. 2013. View Article : Google Scholar : PubMed/NCBI
15	Saxena A and Carninci P: Long non-coding RNA modifies chromatin: Epigenetic silencing by long non-coding RNAs. Bioessays. 33:830–839. 2011. View Article : Google Scholar : PubMed/NCBI
16	Zhang W, Wang L, Zheng F, Zou R, Xie C, Guo Q, Hu Q, Chen J, Yang X, Yao H, et al: Long noncoding RNA expression signatures of metastatic nasopharyngeal carcinoma and their prognostic value. Biomed Res Int. 2015:6189242015. View Article : Google Scholar : PubMed/NCBI
17	Gibb EA, Brown CJ and Lam WL: The functional role of long non-coding RNA in human carcinomas. Mol Cancer. 10:382011. View Article : Google Scholar : PubMed/NCBI
18	Maruyama R and Suzuli H: Long noncoding RNA involvement in cancer. BMB Rep. 45:604–611. 2012. View Article : Google Scholar : PubMed/NCBI
19	Xu T, Su B, Wang C, Wang S, Huang H, Pan Y, Wang D, Wei W, Claret FX and Yang H: Molecular markers to assess short-term disease local recurrence in nasopharyngeal carcinoma. Oncol Rep. 33:1418–1426. 2015.PubMed/NCBI
20	Su YJ, Yu J, Huang YQ and Yang J: Circulating long noncoding RNA as a potential target for prostate cancer. Int J Mol Sci. 16:13322–13338. 2015. View Article : Google Scholar : PubMed/NCBI
21	Weber DG, Johnen G, Casjens S, Bryk O, Pesch B, Jöckel KH, Kollmeier J and Brüning T: Evaluation of long noncoding RNA MALAT1 as a candidate blood-based biomarker for the diagnosis of non-small cell lung cancer. BMC Res Notes. 6:5182013. View Article : Google Scholar : PubMed/NCBI
22	Gao W, Chan JY and Wong TS: Differential expression of long noncoding RNA in primary and recurrent nasopharyngeal carcinoma. Biomed Res Int. 2014:4045672014. View Article : Google Scholar : PubMed/NCBI
23	Zhou X, Cui J, Macias V, Kajdacsy-Balla AA, Ye H, Wang J and Rao PN: The progress on genetic analysis of nasopharyngeal carcinoma. Comp Funct Genomics. 2007:575132007. View Article : Google Scholar
24	Edge SB and Compton CC: The America joint committee on cancer: The 7th edition of the AJCC cancer staging manual and the future of TNM. Ann Surg Oncol. 17:1471–1474. 2010. View Article : Google Scholar : PubMed/NCBI
25	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
26	Chang ET and Adami HO: The enigmatic epidemiology of nasopharyngeal carcinoma. Cancer Epidemiol Biomarkers Prev. 15:1765–1777. 2006. View Article : Google Scholar : PubMed/NCBI
27	Kimura Y, Suzuki D, Tokunaga T, Takabayashi T, Yamada T, Wakisaka N, Yoshizaki T, Murata H, Miwa K, Shoujaku H, et al: Epidemiological analysis of nasopharyngeal carcinoma in the central region of Japan during the period from 1996 to 2005. Auris Nasus Larynx. 38:244–249. 2011. View Article : Google Scholar : PubMed/NCBI
28	Young LS and Dawson CW: Epstein-Barr virus and nasopharyngeal carcinoma. Chin J Cancer. 33:581–590. 2014.PubMed/NCBI
29	Wapinski O and Chang HY: Long noncoding RNAs and human disease. Trends Cell Biol. 21:354–361. 2011. View Article : Google Scholar : PubMed/NCBI
30	Qiu MT, Hu JW, Yin R and Xu L: Long noncoding RNA: An emerging paradigm of cancer research. Tumour Biol. 34:613–620. 2013. View Article : Google Scholar : PubMed/NCBI
31	Quan M, Chen J and Zhang D: Exploring the secrets of long noncoding RNAs. Int J Mol Sci. 16:5467–5496. 2015. View Article : Google Scholar : PubMed/NCBI
32	Rinn JL and Chang HY: Genome regulation by long noncoding RNAs. Annu Rev Biochem. 81:145–166. 2012. View Article : Google Scholar : PubMed/NCBI
33	Yao Y, Li J and Wang L: Large intervening non-coding RNA HOTAIR is an indicator of poor prognosis and a therapeutic target in human cancers. Int J Mol Sci. 15:18985–18999. 2014. View Article : Google Scholar : PubMed/NCBI
34	Zhang J, Zhang P, Wang L, Piao HL and Ma L: Long non-coding RNA HOTAIR in carcinogenesis and metastasis. Acta Biochim Biophys Sin (Shanghai). 46:1–5. 2014. View Article : Google Scholar : PubMed/NCBI
35	Li Z, Zhao X, Zhou Y, Liu Y, Zhou Q, Ye H, Wang Y, Zeng J, Song Y, Gao W, et al: The long non-coding RNA HOTTIP promotes progression and gemcitabine resistance by regulating HOXA13 in pancreatic cancer. J Transl Med. 13:842015. View Article : Google Scholar : PubMed/NCBI
36	Yan TH, Yang H, Jiang JH, Lu SW, Peng CX, Que HX, Lu WL and Mao JF: Prognostic significance of long non-coding RNA PCAT-1 expression in human hepatocellular carcinoma. Int J Clin Exp Pathol. 8:4126–4131. 2015.PubMed/NCBI
37	De Decker HP and Lawrenson JB: The 22q11.2 deletion: From diversity to a single gene theory. Genet Med. 3:2–5. 2001. View Article : Google Scholar : PubMed/NCBI
38	Brinchi V, Curatolo P, Di Franco C and Dallapiccola B: 2 new cases of chromosome 22 with ring structure. Pathologica. 71:3691979.(In Italian). PubMed/NCBI
39	Schneider M and Eliez S: 22q11.2 microdeletion. Arch Pediatr. 17:431–434. 2010.(In French). View Article : Google Scholar : PubMed/NCBI