Analysis of the correlation between the expression of miR-655 and esophageal cancer prognosis

Chang,Peng; Wang,Xiangjun; Zhou,Ya; Hou,Yulong

doi:10.3892/ol.2017.6047

Analysis of the correlation between the expression of miR-655 and esophageal cancer prognosis

Authors:
- Peng Chang
- Xiangjun Wang
- Ya Zhou
- Yulong Hou
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Affiliations: Department of Thoracic and Cardiac Surgery, Xuzhou Cancer Hospital, Xuzhou, Jiangsu 221005, P.R. China
Published online on: April 19, 2017 https://doi.org/10.3892/ol.2017.6047
Pages: 4691-4694
Copyright: © Chang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The correlation between the expression level of microRNA (miR)-655 in esophageal cancer with proliferation, invasion and prognosis was investigated. Reverse transcription-quantitative polymerase chain reaction technique was used to detect the expression level of miR‑655 in esophageal cancer cell lines KYSE410 and EC9706, tumor tissues and normal cancer‑adjacent tissues of 63 cases of patients with esophageal squamous cell carcinoma (ESCC). The expression of miR‑655 was upregulated in ESCC cells through the transfection of miR‑655 mimics, and the influence of miR‑655 on proliferation and invasion ability of ESCC cells was observed. Combined with the clinical data, the correlation between the expression of miR‑655 and the prognosis of esophageal cancer was analyzed. The expression of miR‑655 was downregulated in 2 ESCC cell lines and 73.02% of ESCC tissues. The results showed that the increased expression of miR-655 via transfection of mimics inhibited the proliferation and invasion ability of ESCC cells. Combined with clinical data analysis, it was found that the low‑expression of miR‑655 was related to poorer progression‑free survival. In conclusion, the high‑expression of miR‑655 can inhibit the proliferation and invasion of ESCC, and plays a negative regulation role in the prognosis process of tumor patients. The targeted regulation of miR‑655 can be used as a new treatment method of ESCC.

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