microRNA-497 overexpression decreases proliferation, migration and invasion of human retinoblastoma cells via targeting vascular endothelial growth factor A

  • Authors:
    • Jianjun Li
    • Yinghui Zhang
    • Xiuchao Wang
    • Ruibo Zhao
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  • Published online on: April 24, 2017     https://doi.org/10.3892/ol.2017.6083
  • Pages: 5021-5027
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Abstract

The expression level and roles of microRNA-497 (miR-497) have been frequently reported in previous studies on cancer. However, its expression, function and associated molecular mechanisms in retinoblastoma remain unknown. In the present study, miR‑497 expression levels in human retinoblastoma tissues, normal retinal tissues and retinoblastoma cell lines were determined using reverse transcription‑quantitative polymerase chain reaction. In addition, a Cell Counting Kit‑8 assay, cell migration assay, cell invasion assay, western blot analysis and Dual‑Luciferase reporter assay were used to explore the expression, functions and molecular mechanisms of miR‑497 in human retinoblastoma. It was demonstrated that miR‑497 was significantly downregulated in retinoblastoma tissues and cell lines compared with normal retinal tissues. Ectopic expression of miR‑497 decreased the proliferation, migration and invasion of retinoblastoma cells. Furthermore, VEGFA was verified as a potential direct target of miR‑497 in vitro. Taken together, the results indicate that miR‑497 functions as a tumor suppressor in the carcinogenesis and progression of retinoblastoma via targeting VEGFA. miR‑497 should be investigated as a potential therapeutic target for the treatment of retinoblastoma.
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June-2017
Volume 13 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li J, Zhang Y, Wang X and Zhao R: microRNA-497 overexpression decreases proliferation, migration and invasion of human retinoblastoma cells via targeting vascular endothelial growth factor A. Oncol Lett 13: 5021-5027, 2017
APA
Li, J., Zhang, Y., Wang, X., & Zhao, R. (2017). microRNA-497 overexpression decreases proliferation, migration and invasion of human retinoblastoma cells via targeting vascular endothelial growth factor A. Oncology Letters, 13, 5021-5027. https://doi.org/10.3892/ol.2017.6083
MLA
Li, J., Zhang, Y., Wang, X., Zhao, R."microRNA-497 overexpression decreases proliferation, migration and invasion of human retinoblastoma cells via targeting vascular endothelial growth factor A". Oncology Letters 13.6 (2017): 5021-5027.
Chicago
Li, J., Zhang, Y., Wang, X., Zhao, R."microRNA-497 overexpression decreases proliferation, migration and invasion of human retinoblastoma cells via targeting vascular endothelial growth factor A". Oncology Letters 13, no. 6 (2017): 5021-5027. https://doi.org/10.3892/ol.2017.6083